Human IL-7 ELISA Kit,Absin_specification/price/image

Product Detail
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Product Specification

Theory

The kit is a double-antibody sandwich ELISA method, which is precoated with RNase Inhibitor to capture antibody. After adding the sample, the sample is captured to form antibody-antigen complex, and then HRP-labeled RNase Inhibitor is added to detect antibody. An antibody-antigen-antibody "sandwich" complex was formed. Finally, TMB was added for color development, and the absorbance (OD) value was read at 450nm wavelength after the reaction was terminated. The RNase Inhibitor content in the sample was positively correlated with the OD value.

Description

Detection Principle:

This kit uses double antibody sandwich enzyme-linked immunosorbent assay technology. The specific anti human IL-7 antibody was pre coated on a high affinity microplate. Add the standard, sample to be tested and biotinylated detection antibody into the wells of enzyme plate. After incubation, IL-7 in the sample combines with solid-phase antibody and detection antibody. After washing to remove unbound material, horseradish peroxidase labeled streptavidin HRP was added. After washing, the chromogenic substrate TMB was added to avoid light for color development. The depth of the color response is directly proportional to the concentration of IL-7 in the sample. Stop solution was added to stop the reaction, and the absorbance value was measured at 450 nm wavelength (reference wavelength 570-630 nm)

Detection Type: Double antibody sandwich method

Form: Pre coated 96 well plate

Test Sample Type: Cell supernatant, serum, plasma

Loading Amount: 100 μ L

Kit Components: a copy of pre coated 96 well plate, standard, IL-7 detection antibody, standard dilution, detection buffer, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions

Sensitivity: 0.22 pg/ml

Detection Range: 7.18-500pg/ml

Recovery Range: 80-115%

Storage Method: 2-8 ℃

Standard Curve:

Background:

Interleukin 7 (IL-7)It is a hematopoietic growth factor secreted by bone marrow and thymic stromal cells. It can also be produced by keratinocytes, dendritic cells,
hepatocytes, neurons and epithelial cells, but normal lymphocytes cannot produce IL-7. IL-7 stimulates the differentiation of multipotent hematopoietic stem cells into lymphoid progenitor cells,
and it can also stimulate the proliferation of all lymphoid cells (B cells, T cells, and NK cells)
IL-7 promotes the development of hematologic malignancies (acute lymphoblastic leukemia, T-cell lymphoma). Elevated IL-7 levels have been detected in the plasma of HIV infected patients.
IL-7 can also improve the immune rehabilitation after allogeneic stem cell transplantation&Nbsp

AntBio is a biotechnology group company dedicated to serving life sciences, aiming to help scientists accelerate research and improve work efficiency. AntBio provides comprehensive and high-quality reagent tools for basic research, drug development, and diagnosis, including research grade antibodies, proteins, biochemical reagents, and assay kits. These research tools are widely used in different segments of life science research. The group company currently consists of three brands, Absin, Starter-Bio and UA-Bio.