Product Specification
For researchers, clinical laboratory specialists, and biotech professionals focusing on immunology, mucosal health research, or immune-related disease studies, the Human IgA One-Step ELISA Kit from Antbio Inc. is a high-efficiency, reliable tool designed to simplify and optimize the quantification of human Immunoglobulin A (IgA). As a key antibody in the mucosal immune system, IgA plays a critical role in defending against pathogens at mucosal surfaces (e.g., gastrointestinal, respiratory, and genitourinary tracts) and serves as a vital biomarker for mucosal immune disorders, infectious diseases, or autoimmune conditions—making this kit indispensable for both basic research and preclinical/clinical auxiliary studies.
What sets this kit apart from traditional ELISA solutions is its innovative one-step protocol. By condensing the multi-step, labor-intensive processes of conventional ELISA (including separate antigen-antibody incubation, washing, and signal detection steps) into a single streamlined workflow, it drastically reduces assay time (cutting hands-on operation by up to 50% compared to standard kits) while minimizing human error from repeated sample handling. This ensures consistent, reproducible results that meet the strict standards of academic research and clinical support.
Every component of the kit is manufactured under rigorous quality control standards, utilizing high-specificity polyclonal antibodies against human IgA and premium-grade, stable reagents. This guarantees exceptional sensitivity (with a detection limit as low as pg/mL levels), minimal cross-reactivity with other human immunoglobulins (e.g., IgG, IgM, IgE), and excellent batch-to-batch stability—all critical for generating credible, publishable data. The kit is fully optimized for accurate detection in a range of human biological samples, including serum, plasma, mucosal secretions (e.g., saliva, nasal fluid, intestinal lavage), and cell culture supernatants, enhancing its versatility for diverse research needs.
Backed by Antbio’s decades of expertise in developing application-tailored immunoassay tools, the Human IgA One-Step ELISA Kit is engineered to address the unique demands of mucosal immunology and immune-related research. Whether you’re investigating mucosal immune responses to pathogens, evaluating the efficacy of mucosal vaccines, or studying IgA-related disorders (e.g., IgA nephropathy), this kit delivers the precision, efficiency, and ease of use needed to accelerate your research progress.
To access detailed product specifications (including detection range, sample volume requirements, and assay duration), pricing information, ordering guidelines, and dedicated technical support, visit the official product page via the link below:
| Antigen |
IgA |
| Immunogen |
Recombinant Protein |
| Antibody Type |
Recombinant mAb |
| Reactivity |
Hu |
| Purification |
Protein A |
| Stability & Storage |
2 to 8 °C as supplied. |
Kit
| Precision |
Intra-assay: 4.9%；
Inter-assay: 13.4% |
| Sample type |
Cell culture supernatant, Serum, Plasm |
| Assay type |
Sandwich (quantitative) |
| Sensitivity |
0.014 ng/ml |
| Range |
0.156 ng/mL – 10 ng/mL |
| Recovery |
Cell culture supernatant: 91%
Serum: 103%
Plasm: 101% |
| Assay time |
60 minutes |
| Species reactivity |
Human |
Background
Immunoglobulin A (IgA) is a critical antibody isotype central to mucosal immunity and systemic immune regulation. Structurally, IgA exists in two primary forms: monomeric IgA in serum (predominantly IgA1) and dimeric or polymeric secretory IgA (sIgA) at mucosal surfaces. Each IgA molecule comprises two α-heavy chains and two light chains (κ or λ), forming a flexible Y-shaped structure stabilized by disulfide bonds. The α-heavy chain contains three constant domains (Cα1–Cα3), with the Fc region (Cα2–Cα3) facilitating interactions with Fcα receptors (FcαRI) and the polymeric immunoglobulin receptor (pIgR). Secretory IgA incorporates a J-chain and a secretory component, which enhance its stability in protease-rich mucosal environments. Extensive glycosylation of IgA further modulates its solubility, receptor binding, and resistance to microbial degradation. IgA is the predominant antibody at mucosal surfaces (e.g., respiratory, gastrointestinal, and urogenital tracts), where it neutralizes pathogens, prevents microbial adherence, and regulates commensal microbiota. Secretory IgA mediates immune exclusion by trapping pathogens in mucus for mechanical clearance, while serum IgA contributes to systemic immune regulation by dampening inflammatory responses via FcαRI signaling. Additionally, IgA engages in eosinophil-mediated antiparasitic defense and modulates allergic reactions through interactions with immune cells. Human IgA shares conserved structural motifs with IgA from primates and rodents, particularly in the α-chain constant domains. However, species-specific variations in glycosylation patterns, J-chain association, and secretory component binding necessitate species-specific detection assays. For instance, human IgA1’s unique hinge region, rich in proline and O-glycosylation sites, distinguishes it from murine IgA, which lacks this feature. Applications of the Human IgA ELISA Kit: This OneStep Human IgA ELISA kit enables rapid, accurate quantification of IgA in serum, plasma, or cell culture supernatants. Advantages of this OneStep ELISA Kit Design: Simplified Workflow: Combines sample incubation and detection in a single step, eliminating intermediate washing and reducing hands-on time. High Efficiency: Achieves results within 1 hours, ideal for high-throughput studies or urgent diagnostics. Enhanced Precision: Minimizes procedural variability and cross-contamination risks through optimized reagent formulation. Broad Compatibility: Validated for diverse biological matrices, ensuring flexibility in experimental design. This kit’s streamlined protocol and robust performance make it an indispensable tool for researchers and clinicians seeking reliable IgA quantification in time-sensitive or resource-limited settings.
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