Product Specification
For researchers, preclinical study teams, and immunology specialists focusing on rodent models of allergy, parasitic infection, or immunological disorders, the Rat IgE One-Step ELISA Kit from Antbio Inc. is a high-efficiency, reliable tool designed to streamline the quantification of rat Immunoglobulin E (IgE). As a key antibody class linked to type I hypersensitivity (allergic responses) and immune defense against parasites in rat models—widely used for preclinical testing of anti-allergy therapeutics, studying allergic pathogenesis, and evaluating parasitic immunity—this kit is indispensable for basic immunology research and preclinical drug development.
What sets this kit apart from traditional ELISA solutions is its innovative one-step protocol. By integrating the multi-step, labor-intensive processes of conventional ELISA (including sequential antigen-antibody binding, repeated washing, and separate signal development) into a single streamlined workflow, it reduces hands-on operation time by up to 50% compared to standard kits. This not only boosts laboratory efficiency but also minimizes the risk of human error from repeated sample handling—ensuring consistent, reproducible results that meet the rigorous standards of preclinical research and academic studies.
Every component of the kit is manufactured under strict quality control standards, utilizing high-specificity monoclonal antibodies targeting rat IgE and premium-grade, stable reagents. This guarantees exceptional sensitivity (with a detection limit as low as pg/mL levels), minimal cross-reactivity with other rat immunoglobulins (e.g., IgG, IgA, IgM), and reliable batch-to-batch performance—critical for generating credible, publishable data. The kit is fully optimized for accurate detection in common rat biological samples, including serum, plasma, and cell culture supernatants, making it versatile for diverse research scenarios, from testing allergen-induced IgE responses to evaluating the efficacy of anti-parasitic treatments.
Backed by Antbio’s expertise in developing rodent-focused immunoassay tools, the Rat IgE One-Step ELISA Kit is engineered to address the unique needs of rat model research. Whether you’re investigating the mechanisms of allergic diseases in rodent models, validating IgE as a preclinical biomarker, or studying parasite-induced immune responses, this kit delivers the precision, efficiency, and ease of use needed to advance your work.
To access detailed product specifications (including detection range, required sample volume, and assay duration), pricing details, ordering procedures, and dedicated technical support, visit the official product page via the link below:
Rat IgE One-Step ELISA Kit - Antbio
| Antigen |
IgE |
| Immunogen |
Recombinant Protein |
| Antibody Type |
Recombinant mAb |
| Reactivity |
Rt |
| Purification |
Protein A |
| Stability & Storage |
2 to 8 °C as supplied. |
Kit
| Precision |
Intra-assay: 2.6%；
Inter-assay: 4.2% |
| Sample type |
Cell culture supernatant, Serum, Plasm |
| Assay type |
Sandwich (quantitative) |
| Sensitivity |
0.137 ng/ml |
| Range |
0.39 ng/mL – 25 ng/mL |
| Recovery |
Cell culture supernatant: 88%
Serum: 96%
Plasm: 79% |
| Assay time |
60 minutes |
| Species reactivity |
Rat |
Background
Immunoglobulin E (IgE) is a key antibody isotype integral to allergic reactions and defense against parasitic infections. Structurally, IgE is composed of two heavy chains (ε-chains) and two light chains (κ or λ), forming a monomeric Y-shaped configuration stabilized by disulfide bonds. The ε-heavy chain contains four constant domains (Cε1–Cε4), with the Fc region (Cε3–Cε4) enabling high-affinity binding to FcεRI receptors on mast cells and basophils. Extensive glycosylation of IgE enhances its stability and modulates receptor interactions.
Functionally, IgE initiates type I hypersensitivity responses by crosslinking allergens to FcεRI-bound IgE on mast cells, triggering degranulation and release of inflammatory mediators (e.g., histamine, cytokines). It also contributes to antiparasitic immunity by promoting eosinophil-mediated destruction of helminths.
Cross-Species Homology: Rat IgE exhibits high structural homology with murine and human IgE, particularly in conserved regions of the ε-chain. However, species-specific variations in glycosylation and epitope accessibility necessitate species-specific assays for accurate detection.
Clinical and Experimental Significance of IgE Levels: Elevated IgE: Correlates with allergic diseases (e.g., asthma, atopic dermatitis), parasitic infections, or hypersensitivity disorders. Suppressed IgE: May indicate immunosuppressive therapies, genetic deficiencies (e.g., hyper-IgM syndrome), or immune dysfunction.
Applications of the Rat IgE ELISA Kit: This OneStep Rat IgE ELISA kit is designed for rapid and precise quantification of IgE in serum, plasma, or cell culture supernatants.
Advantages of this OneStep ELISA Kit Design:
Simplified Workflow: Combines sample incubation and detection in a single step, eliminating intermediate washing and reducing hands-on time.
High Efficiency: Achieves results within 1 hours, ideal for high-throughput studies or urgent diagnostics.
Enhanced Precision: Minimizes procedural variability and cross-contamination risks through optimized reagent formulation.
Broad Compatibility: Validated for diverse biological matrices, ensuring flexibility in experimental design.
This kit’s streamlined protocol and robust performance make it an indispensable tool for researchers and clinicians seeking reliable IgE quantification in time-sensitive or resource-limited settings.
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