Product Specification
For researchers, immunologists, and preclinical teams specializing in inflammation research, infectious disease studies, or cancer immunology, the Human IL-8 One-Step ELISA Kit from Antbio Inc. is a high-performance, time-saving tool designed to simplify the accurate quantification of human Interleukin-8 (IL-8). As a potent pro-inflammatory cytokine and key chemoattractant for neutrophils, IL-8 plays a central role in mediating acute inflammatory responses, regulating immune cell recruitment in infections, and driving tumor microenvironment progression—making this kit indispensable for basic research, preclinical drug development, and translational studies.
What differentiates this kit from conventional ELISA systems is its innovative one-step protocol. By condensing the multi-step, labor-intensive processes of traditional ELISA (including sequential antigen-antibody incubation, repeated washing cycles, and separate signal development) into a single streamlined workflow, it cuts hands-on operation time by up to 45% compared to standard kits. This not only enhances laboratory efficiency but also minimizes the risk of human error from repeated sample handling, ensuring consistent, reproducible results that meet the rigorous standards of academic research and biopharmaceutical testing.
Every component of the kit is manufactured under strict quality control protocols, using high-specificity monoclonal antibodies targeting human IL-8 and premium-grade, stable reagents. This guarantees exceptional sensitivity (with a detection limit as low as pg/mL levels), minimal cross-reactivity with other pro-inflammatory cytokines (e.g., IL-6, TNF-α), and reliable batch-to-batch performance—all critical for generating credible, publishable data. The kit is fully optimized for accurate detection in a range of human biological samples, including serum, plasma, cell culture supernatants, and tissue homogenates, making it versatile for diverse research scenarios: from studying sepsis-related acute inflammation to evaluating anti-inflammatory drug efficacy or exploring IL-8’s role in tumor angiogenesis.
Backed by Antbio’s decades of expertise in developing application-focused immunoassay solutions, the Human IL-8 One-Step ELISA Kit is engineered to address the unique challenges of IL-8 research. Whether you’re investigating the molecular mechanisms of inflammatory diseases, validating IL-8 as a disease biomarker, or testing novel anti-inflammatory therapeutics, this kit delivers the precision, efficiency, and ease of use needed to accelerate your work.
To access detailed product specifications (including detection range, required sample volume, and assay duration), pricing information, ordering guidelines, and dedicated technical support, visit the official product page via the link below:
Human IL-8 One-Step ELISA Kit - Antbio
| Antigen |
IL-8 |
| Immunogen |
Recombinant Protein |
| Antibody Type |
Recombinant mAb |
| Reactivity |
Hu |
| Purification |
Protein A |
| Stability & Storage |
2 to 8 °C as supplied.
|
Kit
| Precision |
Intra-assay: 3.3%；
Inter-assay: 4.4% |
| Sample type |
Cell culture supernatant, Serum, Plasm |
| Assay type |
Sandwich (quantitative) |
| Sensitivity |
2.966 pg/ml |
| Range |
3.9 pg/mL – 250 pg/mL |
| Recovery |
Cell culture supernatant: 86%
Serum: 108%
Plasm: 96% |
| Assay time |
60 minutes |
| Species reactivity |
Human |
| NIBSC convert |
The dose response curve of the International Standard (89/520) parallels the OneStep standard curve. To convert sample values obtained with the kit to approximate NIBSC (89/520) units, use the following equation: NIBSC (89/520) approximate value (IU/mL) = 0.001 x OneStep Human IL-8 value (pg/mL). |
Background
Interleukin 8 (IL-8 or chemokine (C-X-C motif) ligand 8, CXCL8) is a chemokine produced by macrophages and other cell types such as epithelial cells, airway smooth muscle cells and endothelial cells. Endothelial cells store IL-8 in their storage vesicles, the Weibel-Palade bodies. IL-8 is initially produced as a precursor peptide of 99 amino acids which then undergoes cleavage to create several active IL-8 isoforms. In culture, a 72 amino acid peptide is the major form secreted by macrophages. Interleukin-8 is a key mediator associated with inflammation where it plays a key role in neutrophil recruitment and neutrophil degranulation. IL-8 has also been implied to have a role in colorectal cancer by acting as an autocrine growth factor for colon carcinoma cell lines or the promotion of division and possible migration by cleaving metalloproteinase molecules. It has also been shown that IL-8 plays an important role in chemoresistance of malignant pleural mesothelioma by inducing expression of transmembrane transporters.
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