Testing Principle：
This kit uses double antibody sandwich ELISA.Specific anti-human IL-33 capture antibody is prepackaged on a high affinity conjugate plate.  The standard substance, the sample to be tested and the detection antibody labeled with biotin were successively added into the HRP-plate well. After fully shaking and mixing, the IL-33 in the sample was placed at room temperature for a 2-hour incubation process. The IL-33 in the sample was combined with the solid phase antibody and the detection antibody.  After washing thoroughly to remove free and unbound ingredients, horseradish peroxidase labeled streptavidin-HRP (SA-HRP) is added.  After washing again, TMB color substrate was added and incubated in dark at room temperature for color development.  The depth of the color reaction was positively correlated with the concentration of IL-33 in the sample.  The termination solution was added to terminate the reaction, and the absorbance value was measured at 450 nm detection wavelength (correction wavelength 570-630 nm) using a microplate analyzer.  

Detection type：Double sandwich enzyme immunoassay

Form: pre-coated 96-well plate

Sample type: Cell culture supernatant, Serum, Plasma

Sample quantity：100 μl

Kit composition：pre-coated 96-well plate,Standards,IL-33 test antibody, Standard Diluent, Test Buffer, TMB Color Substrate, Washing Solution, Stop Solution, SA-HRP, Seal Plate Mask and Instructions  

Sensitivity：2.17 pg/mL

Detection range：31.25-2000 pg/mL

Recovery rate：80-116%

Storage：2-8℃

The standard curve：

Background：

Interleukin 33 (IL-33), also known as NF-HEV and DVS 27, is a member of the IL-1 family with a molecular weight of 30 kDa and is secreted by several cell types, including fibroblasts, mast cells, dendritic cells, macrophages, osteoblasts, endothelial cells, and epithelial cells. IL-33 induces the secretion of type II cytokines by helper T cells, mast cells, eosinophils, and basophils. IL-33 activates extracellular molecules in the NF-κB and MAP kinase signaling pathways by binding to the receptor ST2 and IL-1 receptor helper protein (IL1RAP), which induces the secretion of type II cytokines (such as IL-5 and IL-13) by polarized Th2 cells. To unleash its biological function. After IL-33 is injected, it induces type II cytokines in vivo, which are thought to induce severe pathological changes in mucosal organs.