Desmethylnitrophos O ELISA kit is a competitive enzyme immunoassay for the quantitative analysis of Desmethylnitrophos O in milk, water,vegetarable.
The coupling antigens are coated on the micro-well stripes. The Desmethylnitrophos O in the sample and the coupling antigens coated on the micro-well stripes compete for the anti-Desmethylnitrophos O antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration.
The optical density (OD) value of the sample has a negative correlation with the Desmethylnitrophos O in it. The measurement is made photometrically at 450 nm. The absorption is inversely proportional to the Desmethylnitrophos O concentration in the sample.
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