TEST PRINCIPLE
Sheep Echinococcus Granulosus Ab ELISA Test Kit is an enzyme immunoassay designed to detect the presence of antibody to Sheep Echinococcus Granulosus in the sheep serum. A microtitration format has been configured by coating chlamydia pneumonia antigen in alternating columns on the plate. Upon incubation of the test sample in the coated well, antibody specific to Sheep Echinococcus Granulosus forms a complex with the coated viral antigens. After washing away unbound material from the wells, an anti-porcine horseradish peroxidase conjugate is added that binds to any sheep IgG antibody attached in the wells. In the final step of the assay, unbound conjugate is washed away, and enzyme substrate and a chromogen, TMB,are added to the wells. Stop the reaction by adding stop solution. Within 15 minutes READ the absorbance at 450 nm using 630 nm as the reference wavelength if available.
Enzyme immunoassays for the qualitative of IgG antibodies against Sheep Echinococcus Granulosus in the sheep serum or plasma.
MATERIALS SUPPLIED
1 Microtiter Plate 1 pieces (96 wells)
2 Negative control 1 tube
3 Positive control 1 tube
4 Enzyme Conjugate 1 bottle
5 Washing buffer 2 bottle
6 Substrate A 1 bottle
7 Substrate B 1 bottle
8 Stop solution 1 bottle
9 Sample diluent 2 bottle
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