For researchers dedicated to metabolic studies, endocrine research, or cross-species biological investigations, the Human-Monkey RBP4 One-Step ELISA Kit from Antbio Inc. stands out as a high-efficiency, reliable tool to advance your experimental goals. This specialized kit is designed for the accurate and rapid quantification of Retinol-Binding Protein 4 (RBP4) — a key protein involved in retinol (vitamin A) transport, energy metabolism regulation, and the pathogenesis of metabolic disorders such as insulin resistance and type 2 diabetes — in both human and monkey samples, including serum, plasma, and cell culture supernatants.
Its standout feature lies in the innovative one-step protocol, which revolutionizes the traditional ELISA workflow. By integrating multiple complex steps into a single, streamlined process, this kit significantly reduces assay time (cutting down on hands-on operation compared to standard multi-step ELISA kits) while minimizing the risk of operational errors. This ensures consistent, reproducible results that meet the strict standards of academic research, preclinical trials, and pharmaceutical development. Every component of the kit is produced under rigorous quality control measures, using high-specificity, high-affinity antibodies and premium-grade reagents to guarantee exceptional sensitivity, cross-reactivity control, and batch-to-batch stability — critical for generating credible, publishable data.
Backed by Antbio’s long-standing expertise in developing high-performance life science reagents, the Human-Monkey RBP4 One-Step ELISA Kit is engineered to address the unique needs of cross-species research and metabolic studies. Whether you’re exploring the role of RBP4 in metabolic diseases, investigating interspecies differences in retinol metabolism, or validating biomarkers for endocrine disorders, this kit provides the precision, efficiency, and versatility you need to accelerate your research progress.
| Antigen |
RBP4 |
| Immunogen |
Recombinant Protein |
| Antibody Type |
Recombinant mAb |
| Reactivity |
Hu, Mk |
| Purification |
Protein A |
| Stability & Storage |
2 to 8 °C as supplied.
|
Kit
| Precision |
Intra-assay: 4.6%；
Inter-assay: 5.9% |
| Sample type |
Cell culture supernatant, Serum, Plasma |
| Assay type |
Sandwich (quantitative) |
| Sensitivity |
0.038 ng/ml |
| Range |
0.125 ng/mL – 8 ng/mL |
| Recovery |
Cell culture supernatant: 101%
Serum: 94%
Plasma: 105% |
| Assay time |
60 minutes |
| Species reactivity |
Human, Monkey |
Background
Human RBP4, a lipocalin-family lipid carrier, transports retinol (vitamin A) from hepatic stores to peripheral tissues through its conserved β-barrel structure, circulating in plasma as a complex with transthyretin (TTR) to stabilize delivery. Structural integrity is maintained by disulfide bonds, while glycosylation (e.g., at Asn-95) modulates solubility and interaction dynamics. Monkey RBP4 shares 97% sequence identity with human RBP4, including identical retinol-binding residues (e.g., Thr-67, Gln-98). RBP4 facilitates retinol uptake via the STRA6 receptor, ensuring cellular vitamin A distribution critical for metabolic and immune functions. Elevated RBP4 levels are implicated in insulin resistance, adipose tissue inflammation, and type 2 diabetes pathogenesis by disrupting metabolic signaling pathways. Additionally, it regulates immune responses through retinol-dependent modulation of dendritic cell activity and T-cell function, linking vitamin A metabolism to inflammatory and autoimmune processes. This protein’s dual roles in nutrient transport and disease mechanisms underscore its importance in metabolic health and therapeutic research. Advantages of this OneStep ELISA Kit Design: Human & Monkey RBP4 OneStep ELISA Kit enables rapid (～60 min), sensitive (0.125 ng/mL) detection across human and Monkey samples, with cross-testing of horse and donkey samples, leveraging their structural conservation for translational research in metabolism, nutrition, and therapeutic development targeting insulin resistance or chronic diseases. This kit’s streamlined protocol and robust performance make it an indispensable tool for researchers and clinicians seeking reliable RBP4 quantification in time-sensitive or resource-limited settings.
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