Product Specification
For researchers and laboratory professionals focused on immunology, antiviral research, infectious disease studies, or immune response evaluation, the Human IFN-γ One-Step OptiQuant ELISA Kit from Antbio Inc. is a cutting-edge, high-performance tool designed to redefine the accuracy and efficiency of human Interferon γ (IFN-γ) quantification. As a key cytokine pivotal to antiviral defense, immune activation, and inflammatory regulation, IFN levels serve as critical biomarkers for assessing immune system function, viral infection progression, and the efficacy of immunotherapies—making this kit indispensable for basic research, preclinical trials, and biopharmaceutical development.
What makes this kit a standout choice is its unique combination of one-step workflow convenience and OptiQuant precision technology. Unlike traditional ELISA kits that require tedious multi-step incubations, washes, and manual optimization, the one-step protocol streamlines the entire process, cutting assay time by up to 45% while minimizing human error from repeated sample handling. The integrated OptiQuant technology further elevates performance: it optimizes antibody-antigen binding kinetics and signal detection efficiency, delivering exceptional sensitivity (detection limit as low as pg/mL level) and a broad, linear detection range. This ensures accurate quantification of IFN across varying concentrations—from low levels in baseline immune states to high levels in activated immune responses—without the need for multiple sample dilutions.
Every component of the kit is manufactured under strict ISO-compliant quality control standards, using high-specificity monoclonal antibodies against human IFN-γ and premium-grade, stable reagents. This guarantees minimal cross-reactivity with other cytokines (e.g., IL-2, TNF-α) and consistent batch-to-batch performance, critical for generating reliable, publishable data. The kit is fully optimized for use with common human biological samples, including serum, plasma, and cell culture supernatants, making it versatile for diverse research scenarios—whether you’re studying viral pathogenesis, testing antiviral drug efficacy, or evaluating immune checkpoint inhibitor responses.
Backed by Antbio’s decades of expertise in developing application-tailored immunoassay solutions, the Human IFN-γ One-Step OptiQuant ELISA Kit is engineered to meet the rigorous demands of modern immunological research. It balances ease of use with scientific precision, empowering researchers to accelerate their work without compromising data quality.
To explore detailed product specifications (including exact detection range, sample volume requirements, and assay time), pricing, ordering procedures, and dedicated technical support, visit the official product page via the link below:
Human IFN-γ One-Step OptiQuant ELISA Kit - Antbio
| Antigen |
IFN-γ |
| Immunogen |
Recombinant Protein |
| Antibody Type |
Recombinant mAb |
| Reactivity |
Hu |
| Purification |
Protein A |
| Stability & Storage |
2 to 8 °C as supplied.
|
Kit
| Precision |
Intra-assay: 4.0%；
Inter-assay: 4.5% |
| Sample type |
Cell culture supernatant, Serum, Plasma |
| Assay type |
Sandwich (quantitative) |
| Sensitivity |
2.577pg/ml |
| Range |
15.63 pg/mL – 1000 pg/mL |
| Recovery |
Cell culture supernatant: 93%
Serum: 109%
Plasma: 111% |
| Assay time |
45 minutes |
| Species reactivity |
Human |
| NIBSC convert |
The dose response curve of the International Standard (87/586) parallels the OneStep standard curve. To convert sample values obtained with the kit to approximate NIBSC (87/586) units, use the following equation: NIBSC (87/586) approximate value (IU/mL) = 0.02 x OneStep Human IFNγ value (pg/mL).
|
Background
Interferon-gamma (IFN-γ) is a dimeric cytokine central to innate and adaptive immunity, primarily produced by activated T cells and natural killer (NK) cells. Structurally, IFN-γ exists as a homodimer, with each subunit comprising six α-helical domains stabilized by intra- and interchain disulfide bonds. It signals through a heterodimeric receptor complex composed of IFNGR1 and IFNGR2, initiating JAK-STAT pathway activation. Post-translational glycosylation at asparagine residues (e.g., Asn25 and Asn97 in humans) enhances its stability and modulates receptor interaction dynamics. Immune Regulatory Roles and Therapeutic Cross-Talk of IFN-γ: Interferon-gamma (IFN-γ) orchestrates multifaceted immune responses, exerting critical antiviral and antitumor defense by inducing cellular antiviral states and enhancing tumor immunosurveillance through upregulation of MHC class I/II expression. It activates macrophages to bolster phagocytosis, reactive oxygen species production, and pathogen clearance, while polarizing CD4+ T cells into Th1 subsets to amplify cell-mediated immunity. Despite its protective roles, IFN-γ exhibits a dual nature in inflammation, where excessive levels contribute to autoimmune disorders (e.g., rheumatoid arthritis, multiple sclerosis) and chronic inflammatory pathologies. Therapeutically, IFN-γ synergizes with proinflammatory cytokines like TNF-α and IL-12 to amplify immune activation, while counteracting the immunosuppressive effects of IL-4 and IL-10, highlighting its pivotal yet complex role in balancing immune defense and inflammatory regulation. Advantages of this OneStep ELISA Kit Design: Simplified Workflow: Combines sample incubation and detection in a single step, eliminating intermediate washing and reducing hands-on time. High Efficiency: Achieves results within 45 minutes, ideal for high-throughput studies or urgent diagnostics. Enhanced Precision: Minimizes procedural variability and cross-contamination risks through optimized reagent formulation. Broad Compatibility: Validated for diverse biological matrices, ensuring flexibility in experimental design. This kit’s streamlined protocol and robust performance make it an indispensable tool for researchers and clinicians seeking reliable IFN-γ quantification in time-sensitive or resource-limited settings.
bio-equip.cn
中文
