The used AB SCIEX QTRAP 4500 LC-MS/MS system is a highly reliable, field-proven hybrid mass spectrometer that combines the unparalleled quantitative power of a triple quadrupole with the structural elucidation capabilities of a linear ion trap. Widely regarded as the "workhorse" of the LC-MS/MS world, this platform is engineered for laboratories requiring robust, high-throughput, and sensitive analysis of small molecules in complex matrices. At its core, the system utilizes the legendary Turbo V ion source, which supports both Electrospray Ionization (ESI) and Atmospheric Pressure Chemical Ionization (APCI). This allows for a wide flow rate range (from 5 µL/min to 3 mL/min) and accommodates compounds with varying polarities. Coupled with the QJet ion guide and the AcQuRate pulse-counting detector, the QTRAP 4500 delivers exceptional ion transmission efficiency, a linear dynamic range of over five orders of magnitude, and high signal-to-noise ratios crucial for trace-level quantification.
A defining feature of the QTRAP 4500 is its unique hybrid architecture, which enables the powerful MRM-IDA-EPI (Information Dependent Acquisition) workflow. In a single LC run, the instrument can perform high-sensitivity Multiple Reaction Monitoring (MRM) for precise quantification while simultaneously triggering Enhanced Product Ion (EPI) scans for qualitative confirmation and spectral library matching. This eliminates the need for separate runs to confirm compound identity, significantly boosting productivity and confidence in results, particularly in complex biological, food, or environmental samples. The system is driven by fast eQ electronics, facilitating rapid polarity switching (as fast as 50 ms in MRM mode) and high scan speeds (up to 20,000 Da/s in the ion trap), making it perfectly suited to keep pace with fast UHPLC gradients. Advanced scan modes such as MRM3 (MS/MS/MS) provide additional selectivity for quantifying analytes in high-background matrices, while the optional SelexION+ Differential Ion Mobility Spectrometry (DMS) offers an extra dimension of separation to reduce isobaric interferences.
This refurbished system is a cost-effective powerhouse across diverse analytical sectors. In pharmaceutical R&D and DMPK studies, it is extensively used for the quantitative bioanalysis of drug candidates and metabolites in biological fluids (plasma, urine, tissue homogenates). Clinical and forensic laboratories rely on it for therapeutic drug monitoring (TDM), neonatal screening panels (e.g., amino acids, acylcarnitines), and comprehensive toxicology screens. Furthermore, it is a gold standard in food safety and environmental testing for multi-residue analysis of pesticides, veterinary drugs, mycotoxins, and contaminants like PFAS in water and soil. Controlled via the intuitive Analyst software or SCIEX OS platform, it supports scheduled MRM algorithms and includes compliance-ready features (audit trails, electronic signatures) to meet FDA 21 CFR Part 11 and GMP/GLP requirements. Opting for a properly refurbished and performance-verified used QTRAP 4500 offers laboratories access to premium, publication-grade mass spectrometry capabilities at a significantly reduced capital cost, providing an ideal balance of performance, durability, and value.
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Item
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Specification
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System Type
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Hybrid Triple Quadrupole / Linear Ion Trap (QTRAP 4500)
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Mass Range
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5 – 2,000 m/z (Triple Quad); 50 – 2,000 m/z (Linear Ion Trap)
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Sensitivity (MRM)
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1 pg Reserpine, S/N > 100,000:1 (typical, column injection)
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Scan Speed
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Up to 12,000 Da/s (Q1); Up to 20,000 Da/s (Ion Trap)
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Polarity Switching
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≤ 50 ms (MRM mode)
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Dynamic Range
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> 5 orders of magnitude
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Ion Source
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Turbo V (Standard ESI / APCI)
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Detector
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AcQuRate Pulse Counting Detector
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Ion Guide
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QJet Ion Guide
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Key Scan Modes
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MRM, MRM3, EPI, EMS, MS3, Precursor Ion, MRM-IDA-EPI
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Optional Tech
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SelexION+ Differential Ion Mobility Spectrometry (DMS)
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Data System
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Analyst Software or SCIEX OS (supports 21 CFR Part 11)
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Key Applications
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DMPK/Bioanalysis, Clinical TDM, Food/Env. Residues, Forensics
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