Ethylenediamine Alkoxylate Block Copolymer

•Average Molecular Weight: 18,000

•HLB: >24

S9 Surfactant Pluracare 1307 Prill / Tetronic 1307 Core Applications
(1) Stabilization of Reagent Components

•Targets: Enzymes, antibodies, antigens, fluorescent markers, etc.

•Mechanism:

•Forms micelles (low critical micelle concentration, ~0.001–0.01%) to encapsulate hydrophobic regions, reducing protein denaturation.

•Mitigates interfacial stress damage during lyophilization/reconstitution (synergizes with sugar-based protectants like trehalose).

•Applications:

•ELISA Reagents: Protects horseradish peroxidase (HRP) activity, preventing inactivation from freeze-thaw cycles.

•Chemiluminescence Reagents: Stabilizes luminescent markers (e.g., acridinium esters), reducing background signals.

(2) Anti-Interference (Reduction of Non-Specific Binding)

•Targets: Lipoproteins, cell debris, fibrinogen in serum/plasma.

•Mechanism:

•Hydrophobic PPO chains adsorb lipids, forming micelles to "trap" them and avoid non-specific binding with detection antibodies.

•PEO chains create a hydrophilic barrier, blocking protein adsorption on hydrophobic surfaces (e.g., microplates, magnetic beads).

•Typical Formulations:

•Immunoturbidimetric Reagents: 0.05–0.2% Tetronic 1307 + 0.1% signal enhancer BAP-1 to disperse chylomicrons in lipemic samples.

•Nucleic Acid Extraction Reagents: Utilizes weak cationic properties to bind impurity proteins, improving DNA purity.

(3) Nanomaterial Dispersion

•Targets: Gold nanoparticles (AuNPs), quantum dots, magnetic beads.

•Mechanism:

•PPO chains adsorb onto nanoparticle surfaces, while PEO chains extend outward to provide steric stabilization.

•Star-shaped structure enhances steric repulsion, preventing particle aggregation (superior to linear surfactants like Tween-20).

•Application Examples:

•Lateral Flow Strips: 0.01–0.05% Tetronic 1307 disperses gold-labeled antibodies, improving color uniformity on test lines.

•Homogeneous Immunoassays: Stabilizes fluorescent magnetic microspheres, reducing sedimentation.

(4) Lyoprotection and Reconstitution Optimization

•Function: Prevents inactivation of active ingredients during lyophilization and accelerates reconstitution.

•Mechanism:

•Forms a glassy matrix to minimize ice crystal-induced damage to protein structures.

•Facilitates rapid dispersion and reduces interfacial tension during reconstitution.

•Example Formulation:

•Lyophilized PCR Enzyme Mix: 1% trehalose + 0.1% Tetronic 1307 + 0.05% signal enhancer BAP-1.

Pluracare® and Tetronic® are trademarks of BASF.

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