Molecular Weight: 294

SURFACTANT 10G, chemically named p-Isononylphenoxypoly(glycidol), is a non-ionic surfactant based on a polyglycidol-modified isononylphenol structure. Its unique molecular design provides non-hemolytic properties, low foaming, high enzyme compatibility, and ultra-low interfacial tension, making it an ideal functional additive in in vitrodiagnostic reagents for optimizing fluid performance, stabilizing biomolecules, and ensuring compatibility with complex samples (e.g., whole blood, viscous body fluids).

Mechanism of Action

•Non-Hemolytic Property: Achieves a mild hydrophobic-hydrophilic balance to avoid red blood cell membrane rupture, making it suitable for direct whole blood detection (e.g., lateral flow test strips, microfluidic chips).

•Enzyme Activity Protection: Does not interfere with enzyme active sites, making it compatible with enzyme-based reaction systems (e.g., ELISA, chemiluminescence, PCR), improving detection stability and sensitivity.

•Ultra-Low Dynamic Surface Tension: Rapidly reduces liquid-solid interfacial tension, promoting uniform penetration and spreading of reagents on hydrophobic surfaces (e.g., nitrocellulose membranes, plastic microplates).

•Low Foaming and Anti-Interference: Suppresses foam generation during reagent mixing or automated pipetting, reducing optical signal fluctuations (e.g., in colorimetric or fluorescent detection).

•Broad Chemical Compatibility: Tolerates pH 3–11, high salt concentrations (e.g., 1 M NaCl), and organic solvents (e.g., ethanol, DMSO), adapting to complex reaction systems.

Application Scenarios

•Whole Blood Lateral Flow Strips: Added to sample pad treatment solutions to accelerate whole blood penetration and block red blood cell retention, enhancing detection sensitivity and specificity.

•Enzyme Preservation Solution Stabilizer: Incorporated into enzyme lyoprotectant solutions to reduce enzyme inactivation caused by high temperatures or freeze-thaw cycles (e.g., for Taq polymerase, HRP).

•Latex-Enhanced Immunoturbidimetry: Pre-mixed in latex microparticle suspensions to enhance particle dispersibility and optimize the turbidity signal of antigen-antibody complexes.

•Microfluidic Chip Wetting Agent: Coated onto hydrophobic microchannels to optimize the flow uniformity of blood samples and prevent cell clogging or bubble retention.

•Fluorescent/Chemiluminescent Reagents: Solubilizes hydrophobic fluorescent dyes or luminescent substrates, improving labeling efficiency and signal intensity (e.g., in time-resolved fluorescence detection).

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