Phospholipase A2-activating protein (PLAP) is potentially important in regulating the inflammatory response through its activation of phospholipase A2, which catalyzes the release of arachidonic acid. By screening a human monocyte cDNA library with a mouse Plap cDNA, Chopra et al. (1999) isolated human PLAP cDNAs. The 738-amino acid human PLAP protein predicted by the cDNA sequence has a molecular mass of 80,826 kD; however, immunoblot analysis using antibodies against PLAP detected a 72- to 74-kD protein in human monocyte cell lysates. In mouse macrophages, an antisense Plap oligonucleotide blocked cholera toxin-induced arachidonic acid release, indicating a role for PLAP in the regulation of phospholipase A2 activation.

Mouse Phospholipase A-2-activating protein (PLAA) ELISA Kit employs a two-site sandwich ELISA to quantitate PLAA in samples. An antibody specific for PLAA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPLAA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLAA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLAA bound in the initial step. The color development is stopped and the intensity of the color is measured.

Mouse Phospholipase A-2-activating protein (PLAA) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Find more details at http://www.abbkine.com/product/mouse-phospholipase-a-2-activating-protein-plaa-elisa-kit-kte70736/

Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.