Test Principle
Canine Echinococcus（Ech）Coproantigens ELISA Kit is sandwich-antibody enzyme linked immunosorbent assay designed to detect the presence of antigen to canine Echinococcus Coproantigens in the canine stool. A microtitration format has been configured by coating canine Echinococcus antibody in alternating columns on the plate. Upon incubation of the test sample in the coated well, antigens specific to canine Echinococcus Coproantigens forms a complex with the coated viral antibodies. After washing away unbound material from the wells, an anti-porcine horseradish peroxidase conjugate is added that binds to any canine antigens attached in the wells. In the final step of the assay, unbound conjugate is washed away, and enzyme substrate and a chromogen, TMB, are added to the wells. Stop the reaction by adding stop solution. Within 15 minutes READ the absorbance at 450 nm using 630 nm as the reference wavelength if available.
Enzyme immunoassays for the qualitative of antigens against canine Echinococcus Coproantigens in the stool samples.
Materials Supplied
1 Microtiter Plate 1 pieces (96 wells)
2 Negative control 1 tube
3 Positive control 1 tube
4 Enzyme Conjugate 1 bottle
5 Washing buffer 2 bottle
6 Substrate A 1 bottle
7 Substrate B 1 bottle
8 Stop solution 1 bottle
9 Sample diluent 2 bottle
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