Antibody Purification Resins (GP-ProA) price

Product Detail
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1.Details

The antibody purification Resins (GP-ProA) specifically binds to the Fc region of antibodies through coupled Protein A ligands. High-purity antibodies can be obtained from samples such as ascites, serum, and culture medium through a single-step affinity chromatography purification. The product uses polymethacrylate microspheres as the matrix and recombinant Protein A as the ligand, offering high Physical and chemical stability, low ligand detachment, long lifetime, User-friendly, and is applied to different scenarios.

2.Features

Properties	Performance Specification
Matrix	Polyacrylate
Ligand	r-Protein A
Average Particle Size	70 μm±20μm
Dynamic binding Capacity	30-40mg IgG/ml wet gel
Maximum Pressure Limit	1 MPa
pH Stability	3~ 12 (storage)；2~ 13 (Temporary)
Storage	2~ 10 ℃(20% Ethanol)

Method of Use

The Antibody Purification Resins (GP-ProA) is widely used for the separation and purification of various antibodies. Chromatographic operations typically include column packing, equilibration, sample loading, washing, elution, and regeneration steps.

Equilibration: Equilibrate the chromatography column with 5-10 column volumes (CV) of equilibration buffer (20 mM PB + 0.15 M NaCl, pH 7.0, with appropriate concentration of NaCl to inhibit non-specific adsorption) until the conductivity and pH of the effluent remain constant (consistent with the equilibration buffer).

Sample Loading: The sample buffer should be as consistent as possible with the equilibration buffer. Solid samples can be dissolved and prepared in equilibration buffer; dilute sample solutions can be dialyzed with equilibration buffer; concentrated sample solutions can be diluted with equilibration buffer. To avoid column clogging, samples should be centrifuged or microfiltered (0.45 μm) before loading. The sample loading volume should be determined based on the resins binding capacity and the target protein concentration in the sample.

Washing: After sample loading, continue washing with equilibration buffer until the baseline is reached.

Elution: Elute with elution buffer (20 mM sodium acetate, pH 3.0-4.0 or 0.1 M glycine, pH 3.0), and collect the effluent. After elution, immediately neutralize the collected antibody solution to neutral pH with alkaline buffer (such as 1 M Tris/HCl, pH 9.0) to maintain antibody biological activity and prevent inactivation.

Regeneration and Cleaning in place: After several uses (5-10 cycles, depending on the type and source of raw materials and experimental requirements), the medium requires regeneration and cleaning in place.(1) Wash the column with 0.1 M acetic acid or 20% ethanol for 3-5 CV, then wash to neutrality with buffer for reuse;(2) Alternatively, wash the column with 0.05 M NaOH + 1 M NaCl or 6 M guanidine hydrochloride for 3-5 CV, rinse with 3-10 CV of pure water, then wash to neutrality with buffer for reuse.

Other considerations: During column packing, use, and storage, avoid allowing the column to run dry and prevent air bubbles from entering.

3.Specification

Duoning Biotech is a leading one-stop bioprocess solutions provider from China, committed to offering products and services required for the discovery, development and commercialization production of biologics, including reagents, consumables, instruments, equipment, and services. Leveraging our integrated bioprocess system, we empower our customers to achieve effective, reliable, lower-cost and higher-quality biologics manufacturing processes.