Product Overview
The
MPCS Multi‑Channels Perfusion Culture System is an independently developed high‑throughput cell perfusion platform for laboratory use, available in standard and advanced configurations.
The system offers
2‑channel, 6‑channel, and 10‑channel models to meet diverse experimental throughput requirements.
MPCS is supplied with dedicated standard culture flasks and integrated medium supply/waste collection bottles.
The standard culture flask provides an effective growth area of
25 cm², equivalent to a universal T25 flask, with a standard medium volume of approximately
7.5 mL (medium height: 3–3.5 mm).
The MPCS‑S08 High‑Throughput 8‑Channel Independently Temperature‑Controlled Continuous Perfusion Cell Culture System is especially suitable for temperature‑sensitive research, including:
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Temperature‑dependent cell biological mechanism studies
Precise independent temperature control and high‑throughput replication enable research on cell metabolism, signaling pathways, proliferation, differentiation, and stress responses.
Examples:
- Effects of hypothermia (32–35 °C) on glycolysis in tumor cells
- Phenotypic validation of temperature‑sensitive mutant cell lines
- Regulation of immune cell (T cell / macrophage) activation under mild hyperthermia (37–39 °C)
-
Multivariate coupled high‑throughput drug screening
Ideal for temperature‑dependent and thermosensitive drugs, where single‑temperature screening cannot reflect in vivo conditions.
Example:
Screening thermosensitive chemotherapeutics for tumor microenvironments using combined drug concentration gradients (0–100 μM) and temperature gradients (37–39 °C) to identify optimal temperature‑concentration combinations.
Key Advantages
The MPCS high‑throughput continuous perfusion cell culture system, developed in‑house by SAIGEE Biotech, is designed specifically for laboratory‑scale high‑throughput cell perfusion culture.
It is available in standard and advanced versions with 2‑, 6‑, and 10‑channel configurations for flexible experimental design.
The system includes dedicated standard culture flasks and integrated medium/waste bottles.
The 25 cm² growth area matches standard T25 flasks, and the 7.5 mL working volume supports routine cell culture protocols.
The integrated reservoir design greatly improves experimental convenience and reliability.
The MPCS‑S08 8‑channel independently temperature‑controlled model offers unique advantages:
- Precise independent temperature control for high‑throughput replicate experiments
- Ideal platform for temperature‑related cell biology research
- Enables temperature‑concentration dual‑variable drug screening that closely mimics the in vivo microenvironment
- Supports identification of optimal therapeutic windows for thermosensitive drugs
- Delivers a highly efficient solution for temperature‑sensitive studies
Applications
The SAIGEE Biotech MPCS high‑throughput continuous perfusion cell culture system is widely used in universities, research institutes, and biopharmaceutical laboratories.
It supports applications in cell biology, drug development, regenerative medicine, and other fields.
With multi‑channel flexibility, user‑friendly consumables, and continuous perfusion that mimics the in vivo microenvironment, the system supports long‑term stable culture of many cell types, including CHO, HEK293, and mesenchymal stem cells.
The MPCS‑S08 8‑channel independently temperature‑controlled model is specifically designed for temperature‑sensitive research.
The entire culture process is fully enclosed, with medium supply, waste collection, and the culture chamber physically separated, eliminating risks associated with manual medium changes during long‑term culture.
Typical applications include (but are not limited to):
-
Hypothermia‑induced glycolysis in tumor cells
Study the effects of 32–35 °C hypothermia gradients on glycolysis in MDA‑MB‑231 triple‑negative breast cancer cells using 8 independent temperature channels.
-
Dual‑variable screening of thermosensitive chemotherapeutics
Compare 37 °C, 37.8 °C, and 38.5 °C with 5 drug concentrations (0–100 μM) for tumor microenvironment‑targeted drug screening.
-
Functional analysis of temperature‑sensitive yeast mutants
Investigate cell cycle regulation in
CDC28 temperature‑sensitive yeast using 8 temperature gradients (25–37 °C) under continuous perfusion.
-
Hyperthermia‑mediated immune regulation of T‑cell activation
Evaluate CAR‑T cell activation across 37–39 °C with independent temperature control and real‑time monitoring of CD69, CD25, IL‑2, and IFN‑γ.
-
Temperature optimization for CHO cell antibody production
Compare 37 °C and 31 °C induction strategies for high‑density perfusion culture (>1×10⁷ cells/mL) and antibody yield/quality analysis.
-
Dynamic organoid‑on‑chip culture and drug testing
Patient‑derived colorectal cancer organoids cultured under independent temperature (37–38 °C) and perfusion (0.8–2.5 mL/min) with chemotherapeutic dose gradients.
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