Human APPα ELISA Kit,Absin_specification/price/image

Product Detail
Company Profile

Product Specification

Usage	Specimen Requirements 1. Specimens should be extracted as soon as possible after collection. Extraction procedures should be performed according to relevant literature, and experiments should be performed as soon as possible after extraction. If experiments cannot be performed immediately, specimens can be stored at -20°C, but repeated freezing and thawing should be avoided. 2. Samples containing NaN3 cannot be tested because NaN3 inhibits horseradish peroxidase (HRP) activity. Procedure 1. Dilution of Standard: This kit provides one standard sample at full strength. Users can dilute the sample in a small test tube according to the following chart. 16ng/ml	No. 5 standard	150μl of the original standard was added to 150μl of the standard diluent
8ng/ml	Standard No. 4	150μl of Standard No. 5 was added to 150μl of Standard Diluent
4ng/ml	Standard No. 3	150μl of Standard No. 4 was added to 150μl of Standard Diluent
	Standard No. 2	150μl of Standard No. 3 was added to 150μl of Standard Diluent
1ng/ml	Standard No. 1
Cat	Component name	96T	Serial number	Component name	96T
1	30 times concentrated washing liquid	20ml×1 bottle	6	Developer B solution	6ml×1/bottle
2	Enzyme label reagent	6ml×1 bottle	7	Enzyme-labeled coated plate	12 wells×8 strips	8	Standard product (32ng/ml)	0.5ml×1 bottle
4	Sample diluent	6ml×1 bottle	9	Standard diluent	1.5ml×1 bottle
5	Developer A solution	6ml×1 bottle	10	Sealing film	2 sheets

General Notes1. After removing the kit from the refrigerator, allow it to equilibrate at room temperature for 1 hour before use. If the enzyme-coated plate is opened and not completely used, store it in a sealed bag. 2. Crystals may form in the concentrated wash buffer. Warming in a water bath can aid dissolution during dilution. This will not affect the results. 3. Use a pipette for each sample addition step and frequently calibrate its accuracy to avoid experimental error. The time for each addition should ideally be within 5 minutes. For large numbers of samples, using a dispenser is recommended. 4. Develop a standard curve with each measurement, preferably with replicates. If the analyte content in the sample is too high (the sample OD value is greater than the OD value of the first standard well), dilute the sample a certain number of times (n times) with sample diluent before measurement. When calculating the final dilution, multiply by the total dilution factor (×n×5).

5. The sealing film is for single use only to avoid cross contamination.

6. Please store the substrate in a dark place.

7. Strictly follow the instructions for operation. The test results must be determined based on the readings of the microplate reader.

8. All samples, washing solutions and various wastes should be treated as infectious materials.

9. Components of different batches of this reagent must not be mixed.

Storage Temp.Unopened test kit, sealed and stored at 2-8℃, valid for 6 monthsTest Range0.3ng/ml-18ng/ml

AntBio is a biotechnology group company dedicated to serving life sciences, aiming to help scientists accelerate research and improve work efficiency. AntBio provides comprehensive and high-quality reagent tools for basic research, drug development, and diagnosis, including research grade antibodies, proteins, biochemical reagents, and assay kits. These research tools are widely used in different segments of life science research. The group company currently consists of three brands, Absin, Starter-Bio and UA-Bio.