| Usage | Experimental procedure:
1. Cell preparation:
(1) Plate 96-well or 384-well cell culture plates with appropriate density of cells to be tested. A whiteboard is recommended.
(2) If the experiment is to determine the effect of the compound on cells, add the appropriate concentration of the compound to be detected to the cell plate well. The concentration of organic solvent in the culture medium is kept below 2%. Continue to cultivate for a suitable time according to the experimental needs.
2. Cell viability detection:
(1) Remove CTG-LTM2D cell viability detection reagent 2.0 [Note 1], equilibrate to room temperature (22 ℃-25 ℃) [Note 2], shake gently and mix well.
(2) Take out the cell culture plate to be tested and equilibrate to room temperature (22 ℃-25 ℃).
(3) Add 50μL of cell viability detection reagent 2.0 to 100μL of 96-well plate cells, or 10μL of reagent to 20μL of 384-well plate cells [Note 3].
(4) Shake the experimental plate for 2 minutes to fully lyse the cells, and place it in a dark place to equilibrate the reaction for 10 minutes.
(5) Read the fluorescence signal on the fluorescence plate reader [Note 4]. |
| Synonym | CTG 2.0 cell viability test kit; ATP cell viability detection kit; 2D Cell Viability Detection Kit |
| Description | The ready-to-use cell viability detection kit 2.0 is used to quantitatively detect the ATP content in cells, and the ATP content is directly proportional to the number of viable cells. The reagent can be stored at 4 ℃ for two months after dissolution for the first time, and the reduction of detection signal intensity is < 15%, and there is no loss of function. The reagent has the characteristics of high signal-to-noise ratio, good repeatability and good stability. The ready-to-use formulation reduces the experimental steps of first lysis and then detection, thereby further reducing errors caused by frequent sample loading, and its stable glow signal makes this product especially suitable for high-throughput cell proliferation detection and compound screening.
Product composition:
Luciferase, luciferin and buffer are mixed and filled in 100mL or 500mL brown bottles with the following strengths:
| Specifications | 96-well plate number of detectable wells | Number of detectable 384-well plate wells | | 100mL | 2000 | 10000 | | 500mL | 10000 | 50000 |
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| General Notes | 1. After the first use, the cell viability detection reagent 2.0 can be packaged and stored at-20 °C and below, protected from light. The reagent was repeatedly freezed and thawed 5 times with a signal intensity reduction of < 10% and no loss of function. The reagent was left at room temperature (22 °C) for 10 days or at 4 °C for two months after dissolution for the first time, and the signal intensity was reduced by < 15% without loss of function.
2. The luciferase reaction in cell viability detection reagent 2.0 is sensitive to temperature changes. Reagents and experimental plates need to be equilibrated to room temperature (22 ℃-25 ℃), and the temperature during the test is kept constant (± 1 ℃)
3. Without strict verification, it is not recommended to change the dosage of reaction reagents at will. The volume ratio of cell culture medium and detection reagent should be 2: 1.
4. Cell viability detection reagent 2.0 has different signal attenuation rates for different cell types, and the signal half-life is between 1.5 hr-4hr. It is recommended that the plate reading time should not exceed 2hr. |
| Storage Temp. | Dry Ice Transport. Store at-20 ℃ and below, protected from light, shelf life is 12 months. |
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