Annexin V-EGFP/PI Apoptosis analysis kit,Absin_specification/price/image

Product Detail
Company Profile

Product Specification

Usage

1. Adjust the cell density to about 1 × 10⁶Cells/ml. 2. Remove 0.2 ml of cell suspension from a cell culture flask (2 × 10⁵Cells) were transferred into a clean centrifuge tube. 3. Centrifuge at 1000 g at room temperature for 5 minutes to remove the medium. 4. The cell pellet was gently resuspended with 0.2 ml of pre-cooled PBS solution. 5. Centrifuge at 1000 g for 5 minutes at room temperature to remove PBS. 6. The cell pellet was gently resuspended with 0.2 ml of pre-cooled binding buffer (component C. Binding Buffer). 7. Add 5μl Annexin V-EGFP (component A) and 5μl PI dye solution (component B), and gently mix well. 8. Incubate at room temperature (18-24 °C) for 10-15 minutes in the dark. 9. Alternatively, replenish the volume to 0.4 ml with binding buffer or PBS. 10. Place the sample on ice and protect it from light, and test it as soon as possible. 11. Use flow cytometry or fluorescence microscope for detection and analysis.

Beilstein

Synonym

Annexin V-EGFP/PI Apoptosis Analysis Kit

Description

Annexin V-EGFP/PI cell apoptosis detection kit uses EGFP-labeled Annexin V as a probe to detect the occurrence of early cell apoptosis.

The detection principle is: in normal living cells, phosphotidylserine (PS) is located on the inside of the cell membrane, but in early apoptotic cells, PS flips from the inside of the cell membrane to the surface of the cell membrane and is exposed to the extracellular environment. Annexin-V (Annexin-V) is a Ca2 +-dependent phospholipid binding protein with a molecular weight of 35-36KD, which can bind to PS with high affinity. It can bind to the cell membrane of early apoptotic cells through phosphatidylserine exposed on the outside of the cell.

In addition, Propidium Iodide (PI) is also provided in this kit to distinguish surviving early cells from necrotic or late apoptotic cells. PI is a nucleic acid dye that cannot penetrate the intact cell membrane of normal cells or early apoptotic cells, but can penetrate the cell membrane of late apoptotic and necrotic cells to make the nucleus red. Therefore, when Annexin V is used in combination with PI, PI is excluded from viable cells (Annexin V-/PI-) and early apoptotic cells (Annexin V +/PI-), while late apoptotic cells and necrotic cells are stained double positive by EGFP and PI combination (Annexin V +/PI +).

Product Components:

serial number	Name	20T	50T
A	Annexin V-EGFP	100ul	250ul
B	PI	100ul	250ul
C	Binding Buffer	5ml	10ml

PubChem CID

Storage Temp.

Stored at 2-8 ℃ in the dark from light, the validity period is 6 months. Store at-20 ℃ in the dark from light, shelf life for 12 months or more, avoid repeated freezing and thawing.

AntBio is a biotechnology group company dedicated to serving life sciences, aiming to help scientists accelerate research and improve work efficiency. AntBio provides comprehensive and high-quality reagent tools for basic research, drug development, and diagnosis, including research grade antibodies, proteins, biochemical reagents, and assay kits. These research tools are widely used in different segments of life science research. The group company currently consists of three brands, Absin, Starter-Bio and UA-Bio.