| Usage | 1. For TSA dyes, please use signal amplification reaction solution diluted at 1:100 to prepare the dye working solution (currently used and prepared); DAPI prepared the working solution by diluting it with sterile water at a ratio of 1:100
2 Use of secondary antibody: The reagent kit is anti rabbit secondary antibody, please verify whether the species of the primary antibody match before the experiment. Pika universal secondary antibody, please refer to (# abs50049) |
| Description | There are complex cell composition in tissue microenvironment. The phenotype, state, abundance and distribution of these cells have important biological significance and clinical value. With the help of antibody staining, it can be presented in situ. Immunohistochemical staining is a common technology to study tissue morphology and in situ protein expression. Conventional IHC detection can only show a single index, which is difficult to show the cell composition, state and relationship in the complex tissue microenvironment, and this information is essential for the diagnosis and treatment of disease
tyramine signal amplification technology principle: similar to the DAB color development method of conventional immunohistochemistry, TSA technology also uses HRP labeled secondary antibody. HRP catalyzes the addition of the fluorescein substrate of the system to produce activated fluorescent substrate. The activated substrate can covalently bind with tyrosine on the antigen to make the stable covalently bound fluorescein on the sample. After that, the non covalently bound antibody is washed away by the thermal repair method, and the second round of incubation is carried out after changing a primary antibody, and then changing another fluorescein substrate. In this way, multiple labeling can be realized |
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