Mouse IL-7 ELISA Kit,Absin_specification/price/image

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Product Specification

Description

Detection Principle:

The double antibody sandwich ELISA method was used in this experiment. The anti mouse IL-7 monoclonal antibody is coated on the microplate, the IL-7 in the sample and standard will bind with the antibody fixed on the plate, and the free components will be washed away; Horseradish peroxidase labeled anti mouse IL-7 multi antibody was added to combine with IL-7 bound on the microplate to form an immune complex, and the free components were washed away; Add the substrate solution (chromogenic agent), the color of the solution gradually turns blue, add the stop solution, the solution turns yellow and stops changing. The absorbance was measured with a microplate reader.

Detection Type: Double antibody sandwich method

Form: Pre coated 96 well plate

Detection Sample Type: cell supernatant, serum, Plasma

Loading Amount: 100ul

Kit Components:

Pre coated 96 well plate, standard, anti mouse IL-7 detection antibody, dilution buffer, chromogenic solution (a, b), washing solution, final stop solution, sa-hrp, plate sealing membrane and instructions.

Sensitivity: 8.3 pg/ml

Detection Range: 31.2 - 2000 pg/ml

Recovery Range: 85.0-111.9%

Storage Method: 2-8 ℃

Standard Curve

Background:

IL-7 (interleukin-7)Is a cytokine that plays an important role in lymphocyte differentiation, proliferation, and survival. IL-7 is produced by stromal epithelial cells of the thymus, bone marrow, and intestine. It is mediated by IL-7 r alpha / CD127 and commonγ The receptor complex composed of chain signalsΓ C is also a subunit of the IL-2, -4, -9, -15, and -21 receptors. IL-7 helps maintain all primitive and memory T cells. Moreover, IL-7 is required for T cell dendritic cell interaction. In mice, IL-7 activation by IL-7 r alpha is essential for the development of both T and B cell lineages, whereas in humans, T cells are not required and B cell development is required.

AntBio is a biotechnology group company dedicated to serving life sciences, aiming to help scientists accelerate research and improve work efficiency. AntBio provides comprehensive and high-quality reagent tools for basic research, drug development, and diagnosis, including research grade antibodies, proteins, biochemical reagents, and assay kits. These research tools are widely used in different segments of life science research. The group company currently consists of three brands, Absin, Starter-Bio and UA-Bio.