Detection Principle:

The double antibody sandwich ELISA method was used in this experiment. The anti human GM-CSF monoclonal antibody is coated on the microplate, the GM-CSF in the sample and standard will bind with the antibody fixed on the plate, and the free components will be washed away; Horseradish peroxidase labeled anti human GM-CSF polyclonal antibody was added, and the unbound antibody was washed away; Add substrate solution (chromogenic agent), and the color of the solution is directly proportional to the bound target protein; Add termination solution; The absorbance was measured with a microplate reader.

Detection Type: Double antibody sandwich method

Form: pre coated 96 well plate

Detection Sample Type: cell supernatant, serum, Plasma

Loading Amount: 100ul

Kit Components:

Pre coated 96 well plate, standard, anti human GM-CSF detection antibody, dilution buffer, chromogenic solution (a, b), washing solution, final stop solution, sa-hrp, plate sealing membrane and instructions.

Sensitivity: 0.089-0.740  Pg/ml

Detection Range: 15.6 - 1000 pg/ml

Recovery Range: 85-104%

Storage Method: 2-8 ℃

Standard Curve

Background:

Granulocyte macrophage colony stimulating factor (GM-CSF), also known as csf-2, is a common β chain (β c)The polytrophic 30 kDa member of the cytokine family also includes IL-3 and IL-5. GM-CSF adopts &alpha with two intrachain disulfide bonds-Helical configuration. It is secreted by a variety of activated immune, mesenchymal, and epithelial cell types and circulates in the form of variable glycosylated monomers. During inflammation, it is upregulated in a variety of cell types, including encephalitic T cells, allergen exposed lung endothelial cells, and IgE activated mast cells. Mature human GM-CSF has 54% and 63% amino acid sequence identity with mouse and rat GM-CSF, respectively. The high affinity receptor of GM-CSF is bound by a 50 kDa ligand α Subunit (gm-csfrα)And 120 kDa signal transduction β C composition. According to reports, the stoichiometry of functional GM-CSF receptor is GM-CSF, gm-csfrα And β The 2:2:2 complex of C. It is worth noting that β The C subunit is shared by the receptor complex of IL-3 and IL-5, and IL-5 may pass through gm-csfrα And β C signaling. GM-CSF can also utilize syndecan-2 as a co receptor (14). GM-CSF has many functions. It induces the production of monocytes, neutrophils, and eosinophils from CD34 + stem cell precursors. It can synergize with IL-4 or Flt-3 ligands to induce the development and maintenance of bone marrow and dermal dendritic cells. It also functions as a chemoattractant for neutrophils and dendritic cells. GM-CSF promotes Th1 and Th17 cell-mediated autoimmune inflammation and inflammatory activation of dendritic cells, microglia, alveolar macrophages, and eosinophils. In addition, it synergizes with G-CSF to promote the proliferation and invasion of tumor cells. Human GM-CSF immunoassay is a 3.5-4.5-hour solid-phase ELISA designed to measure the levels of human GM-CSF in cell culture supernatants, serum, and plasma. It contains recombinant human GM-CSF expressed in E. coli and antibodies against recombinant factors. Recombinant human GM-CSF has been shown to be accurately quantified. The results obtained using natural human GM-CSF showed that the linear curve was parallel to the standard curve obtained using the kit standard. These results indicate that the kit can be used to determine the relative mass value of human GM-CSF.