Human IL-22 ELISA Kit,Absin_specification/price/image

Product Detail
Company Profile

Product Specification

Synonym

Cytokine Zcyto18; IL-10-related T-cell-derived inducible factor; IL-21; IL22; IL-22; IL-22IL21; IL-D110; IL-TIF; ILTIFIL-10-related T-cell-derived-inducible factor; IL-TIFMGC79382; interleukin 21; interleukin 22; interleukin-22; MGC79384; TIFa; TIFIL-23; zcyto18

Description

Testing Principle

This kit uses quantitative sandwich enzyme immunoassay technique. A specific anti-human IL-22 antibody is pre-coated on a high affinity plate. Standards, test samples, and biotinylated detection antibodies are added to the wells of the enzyme-labeled plate. After incubation, the IL-22 present in the samples combines with the solid-phase antibodies and the detection antibodies to form immune complexes. After washing to remove unbound material, horseradish peroxidase-labeled streptavidin (HRP) was added. After washing, a chromogenic substrate was added, and the color was developed in the dark. The reaction was stopped by adding a stop solution, and the absorbance was measured at a wavelength of 450 nm (reference correction wavelength of 540 nm or 570 nm).

Detection type: sandwich enzyme immunoassay

Form: pre-coated 96-well plate

Sample type: Cell culture supernatant, Serum, Plasma

Sample quantity: 100ul

Kit composition:

96 well polysyrene microplate coated with capture antibody, standard, detection antibody, 10×reagent diluent, Color reagent (A and B), 25×wash buffer, Stop solution, ELISA plate sealers, specification

Sensitivity: 1.8 pg / mL

Detection range: 31.3 - 2000 pg/mL

Recovery rate: 82-110%

Storage: 2-8 ° C

The standard curve:

Background: IL-22

Il-22 (interleukin-22) is a cytokine that induces ros, IL-6, IL-10, TNF -α, and neutrophil infiltration.It also supports the integrity of the epithelial barrier and induces epithelial cell proliferation during wound healing.The IL-22 signal is transmitted through a receptor complex consisting of IL-22R and IL-10R.Il-10 R beta is a common component of IL-10, IL-26, IL-28 and IL-29 receptor complexes.Il-22 binds to IL-22BP and blocks the interaction between IL-22 and IL-22R.

AntBio is a biotechnology group company dedicated to serving life sciences, aiming to help scientists accelerate research and improve work efficiency. AntBio provides comprehensive and high-quality reagent tools for basic research, drug development, and diagnosis, including research grade antibodies, proteins, biochemical reagents, and assay kits. These research tools are widely used in different segments of life science research. The group company currently consists of three brands, Absin, Starter-Bio and UA-Bio.