Recombinant Enterokinase, His tag (High specific activity),UA BIOSCIENCE_specification/price/image

Product Detail
Company Profile

Product Specification

Species	Human
Synonyms	Enteropeptidase, ENTK, hEK, hEK-His
Expression System	E.coli
Molecular Weight	28 kDa (Reducing)
Purity	＞95% by SDS-PAGE
Storage Buffer	20 mM Tris-HCl, 200 mM NaCl, 2 mM CaCl2 (pH 7.4 @ 25°C)
Stability & Storage	Store at -25 ~ -15℃ for 2 years
Reference	1.Melicherová, Kristína,Krahulec, Ján, afránek, Martin, et al. Optimization of the fermentation and downstream processes for human enterokinase production in Pichia pastoris[J]. Appl Microbiol Biotechnol, 2017, 101(5):1927-1934. 2. Gasparian M E, Ostapchenko V G, Schulga A A, et al. Expression, purification, and characterization of human enteropeptidase catalytic subunit in Escherichia coli[J]. Protein Expression & Purification, 2003, 31(1):133-139.

Background

Enterokinase is a specific protease that cleaves after lysine at its cleavage site Asp-Asp-Asp-Asp-Lys. It will sometimes cleave at other basic residues, depending on the conformation of the protein substrate. This product is a high-purity, high-specific activity enterokinase prepared using a recombinant E.coli. It boasts a broad range of applicability (operating temperature: 4-45°C; pH range: 4.5-9.5) and retains partial activity in the presence of various detergents and denaturants.

Components

Storage Solution: 5 U/μL hEK-His in20 mM Tris-HCl, 200 mM NaCl, 2 mM CaCl2, pH 7.4 @ 25°C

Protocol

Optimal incubation times and enzyme concentrations must be determined empirically for a particular substrate. Typical reaction conditions are as follows:
Combine 500 ug of sample with reaction buffer
Recommended Reaction Buffer: 20 mM Tris-HCl, 50 mM NaCl, 2 mM CaCl2 (pH 8.0)
Add 1 U of Enterokinase light chain
Incubate at 25°C for 16 hours

Guidelines

1. Enterokinase is inhibited by high salt concentrations. For optimal activity NaCl concentration should be 50 mM or less. The pH of the buffer should be between 6 and 9. The enzyme requires 2 mM Calcium for activity.

Unit Definition

One unit is defined as the amount of enzyme required to cleave 500 µg of substrate to 95% completion in 16 hours at 25°C.

AntBio is a biotechnology group company dedicated to serving life sciences, aiming to help scientists accelerate research and improve work efficiency. AntBio provides comprehensive and high-quality reagent tools for basic research, drug development, and diagnosis, including research grade antibodies, proteins, biochemical reagents, and assay kits. These research tools are widely used in different segments of life science research. The group company currently consists of three brands, Absin, Starter-Bio and UA-Bio.