RNase H,UA BIOSCIENCE_specification/price/image

Product Detail
Company Profile

Product Specification

Synonyms	Ribonuclease HI、RNase HI、Ribonuclease H、RNase H
Amino Acid Sequence	/
Molecular Weight	19 kD
Purity	＞95% by SDS-PAGE
Conjugation	Unconjugated
Tag	His Tag
Physical Appearance	Liquid
Storage Buffer	50 mM KCl、10 mM Tris-HCl、0.1 mM EDTA、1 mM DTT、200 µg/ml BSA 50% Glycerol（pH 7.4 @ 25°C）
Reconstitution	/
Stability & Storage	Store at -25 ~ -15℃ for 2 years
Reference	[1] Chan S H , Whipple J M , Dai N ,et al.RNase H-based analysis of synthetic mRNA 5' cap incorporation[J].RNA (New York, N.Y.), 2022, 28(8):1144-1155. [2] Ilina T V , Brosenitsch T , Sluis-Cremer N ,et al.Retroviral RNase H: Structure, mechanism, and inhibition[J]. 2021.

Background

Endonuclease that specifically degrades the RNA of RNA-DNA hybrids. RNase H participates in DNA replication; it helps to specify the origin of genomic replication by suppressing initiation at origins other than the oriC locus; along with the 5'-3' exonuclease of pol1, it removes RNA primers from the Okazaki fragments of lagging strand synthesis; and it defines the origin of replication for ColE1-type plasmids by specific cleavage of an RNA preprimer. Involved in production of retron derived msDNA (a branched RNA linked by a 2',5'-phosphodiester bond to a single-stranded DNA).

Components

Storage Solution: 5U/μl RNase H、50 mM KCl、10 mM Tris-HCl、0.1 mM EDTA、1 mM DTT、200 µg/ml BSA 50% Glycerol（pH 7.4 @ 25°C） 10*Reaction Buffer: 500 mM Tris-HCl、750 mM KCl、30 mM MgCl2、100mM DTT(pH 8.3 @ 25°C)

Protocol

Set-up a typical reaction as follows 1）Add the following components in sequence 2）Incubate at 37°C for 20 minutes This reaction can be scaled up according to experimental needs.

Guidelines

1、Both metal ion chelating agent and sulfhydryl sealer can inhibit RNase H activity 2、Heating at 65℃ can be inactivated for 10min

Unit Definition

One unit is the amount of enzyme required to hydrolyze 1 nmol nucleotide from a 20 pmol fluorescent-labeled 50 bp RNA-DNA hybridization chain at 37℃ for 20 minutes in a 50 µl reaction system.

AntBio is a biotechnology group company dedicated to serving life sciences, aiming to help scientists accelerate research and improve work efficiency. AntBio provides comprehensive and high-quality reagent tools for basic research, drug development, and diagnosis, including research grade antibodies, proteins, biochemical reagents, and assay kits. These research tools are widely used in different segments of life science research. The group company currently consists of three brands, Absin, Starter-Bio and UA-Bio.