RNase Ⅲ,UA BIOSCIENCE_specification/price/image

Product Detail
Company Profile

Product Specification

Synonyms	Ribonuclease 3,Ribonuclease III,RNase III
Amino Acid Sequence	/
Molecular Weight	70 kD
Purity	＞95% by SDS-PAGE
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	500 mM NaCl、10 mM Tris-HCl、0.5 mM EDTA、1 mM DTT、50% Glycerol （pH 8 @ 25°C）
Reconstitution	/
Stability & Storage	Store at -25 ~ -15℃ for 2 years
Reference	[1] Nicholson AW. Ribonuclease III mechanisms of double-stranded RNA cleavage. Wiley Interdiscip Rev RNA. 2014 Jan-Feb;5(1):31-48. [2] Wu CX, Xu XJ, Zheng K, Liu F, Yang XD, Chen CF, Chen HC, Liu ZF. Characterization of ribonuclease III from Brucella. Gene. 2016 Apr 1;579(2):183-92. [3] Sun, Weimei et al. Catalytic mechanism of Escherichia coli ribonuclease III: kinetic and inhibitor evidence for the involvement of two magnesium ions in RNA phosphodiester hydrolysis. Nucleic Acids Research 33 (2005): 807 - 815.

Background

The processing of double-stranded(ds) RNA by RNase III family members is an essential step in the maturation and decay of coding and noncoding RNAs, including miRNAs and siRNAs. RNase III family members share a unique fold (RNase III domain) that can dimerize to form a structure that binds dsRNA and cleaves phosphodiesters on each strand, providing the characteristic 2 nt, 3'-overhang product ends. RNase III converts long double-stranded RNA into a heterogeneous mix of short (18–25 bp) interfering RNAs (siRNA).

Components

Storage Solution: 2U/μl RNase Ⅲ、500 mM NaCl、10 mM Tris-HCl、0.5 mM EDTA、1 mM DTT、50% Glycerol（pH 8 @ 25°C） 10*Reaction Buffer: 500 mM Tris-HCl、1 mM DTT 50 mM NaCl (pH 7.5 @ 25°C) 10*EDTA: 500 mM 10*MnCl2: 200 mM

Protocol

Set-up a typical reaction as follows 1）Add the following components in sequence 2）Incubate at 37°C for 20 minutes This reaction can be scaled up according to experimental needs.

Guidelines

Please avoid repeated freeze-thaw cycles.

Unit Definition

One unit is the amount of enzyme required to digest 1 μg of dsRNA to siRNA in 20 minutes at 37°C in a total reaction volume of 50 μl.

AntBio is a biotechnology group company dedicated to serving life sciences, aiming to help scientists accelerate research and improve work efficiency. AntBio provides comprehensive and high-quality reagent tools for basic research, drug development, and diagnosis, including research grade antibodies, proteins, biochemical reagents, and assay kits. These research tools are widely used in different segments of life science research. The group company currently consists of three brands, Absin, Starter-Bio and UA-Bio.