DNase Ⅰ,UA BIOSCIENCE_specification/price/image

Product Detail
Company Profile

Product Specification

Species	Bovine Pancreatic
Synonyms	DNASE,Deoxyribonuclease-1
Expression System	E.coli
Molecular Weight	72kDa (Reducing)
Purity	>95% by SDS-PAGE&HPLC
Conjugation	Unconjugated
Tag	His Tag, MBP Tag
Physical Appearance	Liquid
Storage Buffer	10 mM Tris-HCl, 2 mM CaCl2 ,50% Glycerol，(pH 7.6, 25°C)
Stability & Storage	Store at -25 ~ -15℃ for 2 years
Reference	[1] Vanecko S, Laskowski M. Studies of the Specificity of Deoxyribonuclease I[J]. Journal of Biological Chemistry, 1961, 236(236):3312-6. [2] Kienzle N, Young D, Zehntner S, et al. DNaseI treatment is a prerequisite for the amplification of cDNA from episomal-based genes[J]. Biotechniques, 1996, 20(4):612-6. [3] Michael,R, Green, et al. Human β-globin pre-mRNA synthesized in vitro is accurately spliced in xenopus oocyte nuclei[J].Cell, 1983, 32(3):681-694.

Background

DNase I (Deoxyribonuclease I), can digest single or double-stranded DNA to produce mono deoxynucleotides or single or double-stranded oligo deoxynucleotides, its optimal working pH range is 7-8. DNase I activity is dependent on Ca2+ and can be activated by other bivalent metal ions such as Mg2+, Mn2+, Zn2+, etc. In the presence of Mg2+, the enzyme can randomly recognize and cut any site on any strand of DNA. In the presence of Mn2+, two strands of DNA can be cut at the same site to form sticky ends with flat ends or 1-2 nucleotides protruding.

Components

Storage Solution: 2 U/ul DnaseⅠ、10mM Tris-Hcl、2mM CaCl2、50%Glycerol (pH7.6, 25℃)

10*Reaction Buffer: 100mM Tris-Hcl、25mM MgCl2、5mM CaCl2 (pH7.6, 25℃)

Protocol

This step is suitable for linearization of 1 μg DNA (≥100 nt) and can be scaled up according to experimental needs.

1）Add the following components in sequence

Components	Volume
Plasmid DNA	1μg DNA
10*Reaction Buffer	2μl
DnaseⅠ (2U/μl)	1μl
RNase-free ddH2O	Up to 50μl

2）Incubate at 37°C 1 h.

Guidelines

1. EDTA should be added to a final concentration of 5 mM to protect RNA from being degraded during enzyme inactivation 2. Please avoid repeated freeze-thaw cycles

Unit Definition

One unit is defined as the amount of enzyme which will completely degrade 1 µg of pBR322 DNA in 10 minutes at 37°C in DNase I Reaction Buffer.

AntBio is a biotechnology group company dedicated to serving life sciences, aiming to help scientists accelerate research and improve work efficiency. AntBio provides comprehensive and high-quality reagent tools for basic research, drug development, and diagnosis, including research grade antibodies, proteins, biochemical reagents, and assay kits. These research tools are widely used in different segments of life science research. The group company currently consists of three brands, Absin, Starter-Bio and UA-Bio.