| Description |
Basement membrane is a layer of stromal membrane on the basal surface of epithelial cells in animals. Organogel is a Matrigel formed by basement membrane components extracted from mouse tumor tissues. The main components of the Matrigel are laminin, collagen IV, heparan sulfate proteoglycans (Kleinman et al. 1986). At the same time, this Matrigel also contains a variety of growth factors, such as epidermal growth factor EFG, platelet-derived growth factor PDGF, nerve growth factor NGF, basic fibroblast growth factor FGF-2, transforming growth factor beta B, and insulin-like growth factor ilgf (vukicevic et al.1992)
Product characteristics: This product is in 4° It is liquid under the condition of C, but when heated to 37° Gel state at C. After the Matrigel solidified, it was put back into 4-deg; C overnight, the Matrigel can liquefy again. |
| General Notes |
This matrix gel starts to solidify into a gel when the temperature exceeds 10°C, so it is recommended to handle the matrix gel on ice whenever possible. When passaging organoids to avoid enzyme impact, you can gently pipette the matrix gel using cold pre-chilled basal culture medium at 4°C to release the organoids from the matrix gel. |
| Storage Temp. |
It is suggested that users should separate the products according to the single dosage after the first melting, -80° C refrigerator, 2 years |
| Applications |
Higher protein concentration means higher matrix stiffness and scaffold integrity. Suitable for in vivo cell delivery applications, it can improve cell implantation effects and promote solid tumor formation. |
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