Form |
The product is liquid at 4 ° C, but it is gel when heated to 37 ° C. After the matrix adhesive solidifies, it can be re liquefied by placing it back at 4 ° C overnight. |
Description |
Basement membrane is a layer of stromal membrane on the basal surface of epithelial cells in animals. Organogel is a Matrigel formed by basement membrane components extracted from mouse tumor tissues. The main components of the Matrigel are laminin, collagen IV, heparan sulfate proteoglycans (Kleinman et al. 1986). At the same time, this Matrigel also contains a variety of growth factors, such as epidermal growth factor EFG, platelet-derived growth factor PDGF, nerve growth factor NGF, basic fibroblast growth factor FGF-2, transforming growth factor beta B, and insulin-like growth factor ilgf (vukicevic et al.1992)
Product characteristics: This product is in 4° It is liquid under the condition of C, but when heated to 37° Gel state at C. After the Matrigel solidified, it was put back into 4-deg; C overnight, the Matrigel can liquefy again. |
General Notes |
The temperature of the Matrigel is higher than 10 deg; C will start to solidify into glue, so try to operate the Matrigel on ice. In order to avoid the influence of enzyme on organoids, 4-deg can be used directly when organoids are passaged; C precooled basal medium can release organoids from Matrigel by blowing Matrigel slowly |
Storage Temp. |
It is suggested that users should separate the products according to the single dosage after the first melting, -80° C refrigerator, 2 years |
Applications |
Suitable for experiments that require color detection (such as colorimetric and fluorescent methods). |