SPPS reactor is a kind of glass nutsche filter reactor designed to the repetitive chemical reactions required in solid-phase peptide synthesis.

It combines precise control of reaction conditions with efficient mixing and washing of solid phase (resin) to produce peptides.

Basic Technical Specifications: 

1. Volume: 1L, 2L, 3L, 5L, 10L, 20L, 30L, 50L, 100L, for the small laboratory research scale to pilot plant kilo-lab scale.

2. Vessel: Single wall, double wall or triple wall available

3. Working pressure: Full vacuum to 0.05/0.1Mpa

4. Working temperature: -80°C ~ +220°C

5. Wetted parts: Borosilicate glass 3.3, PTFE

6. Filter device: PTFE, glass or stainless-steel, pore size 0.1um to 200um

In the fields of biochemistry and pharmaceutical research, solid-phase peptide synthesis (SPPS) is an essential technology that allows for the effective synthesis of peptides.

Central to this process is the SPPS reactor, a specialized equipment that facilitates the synthesis of peptides through synthesis cycles.

What is Solid-Phase Peptide Synthesis(SPPS) ?

Solid-phase synthesis is a common technique for peptide synthesis. Usually, peptides are synthesised from the carbonyl group side (C-terminus) to amino group side (N-terminus) of the amino acid chain in the SPPS method, although peptides are biologically synthesised in the opposite direction in cells. In peptide synthesis, an amino-protected amino acid is bound to a solid phase material or resin (most commonly, low cross-linked polystyrene beads), forming a covalent bond between the carbonyl group and the resin, most often an amido or an ester bond. Then the amino group is deprotected and reacted with the carbonyl group of the next N-protected amino acid. The solid phase now bears a dipeptide. This cycle is repeated to form the desired peptide chain. After all reactions are complete, the synthesised peptide is cleaved from the bead.

The protecting groups for the amino groups mostly used in the peptide synthesis are 9-fluorenylmethyloxycarbonyl group (Fmoc) and t-butyloxycarbonyl (Boc). A number of amino acids bear functional groups in the side chain which must be protected specifically from reacting with the incoming N-protected amino acids. In contrast to Boc and Fmoc groups, these have to be stable over the course of peptide synthesis although they are also removed during the final deprotection of peptides.

For more information, please visit us:
SPPS Reactor

https://njhjchem.com/product/glass-nutsche-filters/custom-spps-synthesis-reactor-1l-100l/