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Home >Products> Synthesizers | Sequencers | Cell Analysis >DNA / Organic / Polypeptide Synthesizers> Nucleic acid synthesizer（AUTOOLIGO600）
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- Nucleic acid synthesizer（AUTOOLIGO600）
- Product Detail
- Company Profile
- UTOOLIGO is a flexible and intuitive full-automatic oligonucleotide synthesizer, which can be used for 1 μ The rapid synthesis of mol-50mmol nucleic acid sample is suitable for clinical research, medical development and the synthesis of molecular diagnostic probes.
AUTOOLIGO comes in four models:
● AUTOOLIGO, synthesis capacity 10~100 μ mol
● AUTOOLIGO100, synthetic capacity 50 μ mol~9mmol
● AUTOOLIGO150, synthesis capacity 150 μ mol~12 mmol
● AUTOOLIGO600, synthesis capacity 1mmol-50mmol
Unique AutoOligo adopts a high-precision metering pump drive system, which is compatible with reagents for nucleic acid synthesis. The adoption of flow solid phase column synthesis technology can significantly reduce reagent consumption, ensure the accurate control of reaction speed and contact time, and at the same time, the synthesis efficiency is higher, facilitating linear amplification, so as to obtain cost-effective and high-quality synthesis results.
AUTOOLIGO can use up to 14 kinds of monomers at the same time, and continuously and automatically perform the synthesis process on 1 or 3 or 8 columns.
The CD System workstation has a powerful data management system to provide you with a complete and reliable nucleic acid synthesis method development platform.
● High precision metering pump, flow path resistant to organic reagents;
● Multiple column positions are optional;
● High degree of automation and low reagent consumption;
● Monomer solution recycling;
● Sequential synthesis;
● High production capacity: one synthetic cycle can be completed within 20 minutes at the earliest;
● Online monitoring of ultraviolet, conductivity signal and pressure;
● The software interface is friendly and the method can be edited flexibly;
● Comply with relevant regulations and requirements of FDA and USP;
Equipment parameters（AUTOOLIGO 600）
| No. | project | parameter |
| 1 | Synthesis scale | 1mmol-50mmol |
| 2 | Number of pumps | 3 |
| 3 | Pump type | Plunger pump |
| 4 | Operating flow rate | 4~1200ml/min |
| 5 | Maximum pump pressure | 20bar |
| 6 | Pressure flow rate control | yes |
| 7 | Linear flow rate control | yes |
| 8 | valve | 9 (expandable to 11) |
| 9 | Number of monomer entrances | 11 (expandable to 19) |
| 10 | Number of solvent inlets | 8 |
| 11 | Number of column positions | 1 |
| 12 | Post column pressure | 1 |
| 13 | Post column UV detector | 1 |
| 14 | Conductivity temperature integration module | 1 |
| 15 | Export quantity | 4 |
| 16 | Air supply module | 1 |
| 17 | External communication interface | 2 |
| 18 | Input pressure of external gas | 0.5-0.7bar |
| 19 | Equipment gas input pressure | 0.35-0.5bar |
| 20 | Material of liquid pipeline | FFKM/PEEK/FEP/stainless steel/ceramic |
| 21 | Frame, cabinet and support materials | SUS316L |
| 22 | Dimensions (WxHxD) | 1200*700*1750mm |
| 23 | Power Supply | 220V |
Case 2: Synthesis of DNA 63mer oligonucleotide fragments:
Purpose:
Synthesize one ssDNA strand, 63mer (not listed due to the requirements of sequence confidentiality), with the synthesis amount of 174 μ Mol, the purity requirement after purification is more than 95%.
Room temperature 25 ℃, humidity 60%
● Experimental instruments and reagents:
1. Nucleic acid synthesizer: AUTOOLIGO100
2、HPLC：1000HPLC
3. Protein purifier: Autopure100
4. Mass spectrometer: ThermoFisher LTQ
5. Synthetic column: 6.3ml
6. Carrier: Nitto H09,002
7. Reagents: deprotective agent, activator, oxidant, thio reagent, CAPA, CAPB, anhydrous acetonitrile, 20% DEA, ammonia.
8. Monomer: A, C, G, T, U.
● Results and analysis
The purity of the crude product was 46.3% and 97.8% after one-step reverse phase chromatography. Finally, the molecular weight of the target product is 19344, which is in line with the theoretical molecular weight of the target product (19342.64)
Purpose:
Synthesis of one RNA chain, 76 mer, synthesis amount 70 μ mol
Instrument: AUTOOLIGO Result analysis:
The initial synthesis purity was 69.61%, and the purity was 93.93% after one-step purification. The molecular weight detected by mass spectrometry is 25133, which is consistent with the theoretical value (25131.2).
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Suzhou COSTONES Instruments Co., Ltd. (COSTONES) is an instrument company focusing
on separation and purification. It is an equipment supplier and service provider
for biopharmaceutical, cell therapy and gene testing. It is committed to providing
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The company has a perfect service system and executive power. It interprets the
user experience from the four aspects of technical communication, scheme design,
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in every link until it is recognized by customers.
For a long time, we have maintained good cooperative relations with domestic and
foreign suppliers. The main products include protein purification system,
compressible chromatographic column, ultrafiltration system, chromatographic
ultrafiltration accessories, etc., and provide maintenance services and customized
product services;
At present, Suzhou quarrying has provided high-quality services for
biopharmaceutical, cell therapy, research institutes and scientific research users.
It has carried out in-depth cooperation with customers and become a reliable partner
for customers to achieve win-win results.
We take quality first as the standard, quickly respond to customer needs, help
customers solve problems, improve efficiency, and take improving core
competitiveness for customers as our service tenet. We are willing to realize our
own value while creating value for customers and society through our unremitting
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