BOLV  FERMENTER  Cell Culture Bioreactor

BioCore QF系列

APPLICATIONS:

MAMMALIAN cell, INSECT CELL suspension cell culture, microcarrier attachment cell culture vaccine, recombinant protein and antibody development .

BioCore QC bench cell Bioreactor uses the latest mechanical processing and manufacturing processes, meet customer requirements for cell suspension culture and adherent cell culture, and comply with CGMP, FDA requirements. BIOCORE QC series Bioreactor are available in 2L, 5l, 7.5 l and 15L for batch and continuous culture of animal, insect and plant cells Equipped with a PITCHED BLADE, fixed bed agitator, suitable for suspension cell, micro-carrier and plate carrier culture.

Features:

10-inch display and Siemens PLC system as the control system, reliable and stable. PH-controlled acid-base addition and conversion can automatically judge and switch the growth state in cell culture by using time to prevent the mutual addition of acid-base produced by overcharging and effectively prevent the increase of osmotic pressure. Dissolved Oxygen Control accuracy, using self-designed PID control function, the deviation of ± 1% .

The culture temperature is precisely controlled by the combination of Metal Electric Heating Diaphragm and PID control.

High Quality Components (Mettler Hamilton electrode, Panasonic Schneider Motor, Siemens Controller, Watson-Marlow peristaltic pump, etc.) are selected to ensure reactor quality. Different types of agitator (push agitator, lift agitator and fixed bed agitator) can be selected to meet the requirements of cell culture.

The parameters are controlled by hardware and software filtering to ensure the stability and reliability of the parameters.

316L stainless steel can cover and components after electric polishing with special treatment, can be anti-corrosion, easy to clean.

The parameter adopts the function of formula, which is convenient for storing and calling the parameter.

Watson-Marlow 114 programmable adjustable speed peristaltic pump can be added in acid or Alkali, feed, defoaming between any designation.

Simple and convenient installation, plug and ventilation can be used, parameters can be displayed graphically, simple and easy to learn. Tank interface and joint, micro-carrier culture and free suspension culture, propulsive impeller,.

performance characteristics

★ With Low Shear Force, mixing effect is good, meet the free suspension and micro-carrier adherent cell culture.

With continuous harvest and perfusion of the Liquid Exchange Tube, can meet the continuous culture conditions of cell density 10 million/ml volume flow requirements.

Bubble-free ventilators eliminate the effects of bubbles on cell culture and processes. Optional micro-pore ventilation or small-pore ventilation.

The interface reserve quantity is sufficient, satisfies each kind of cultivation request.

Agitation speed can be cascaded in different ways to meet the requirements of different incubation time.

The control system gives an automatic alarm for failure and limitation, which is convenient for maintenance and use.

It can realize multi-stage cultivation and rapidly enlarge to production scale.

The plate carrier culture fixed bed stirring paddle fixed bed Bioreactor was used to simulate the static growth conditions of the cells, providing an environment similar to that of spin bottle culture, and for the culture conditions, for example, PHDO temperature can be effectively controlled to provide a good growth environment for cell growth, to achieve the goal of high cell density culture, to create conditions for increasing yield.

Performance characteristics:

When the cells are cultured in a fixed bed, the cells enter into a sheet-like polyester sheet and are in a relatively static state.

Various nutrients are transferred to the growth cells through the flow of the liquid, eliminating the damage caused by the shearing force of the stirring paddle, the lamellar carrier which is beneficial to the growth of cells adopts a three-dimensional structure, which is beneficial to the transfer and exchange of nutrients and oxygen in the deep micro-pores for direct aeration, and oxygen transfer is sufficient to ensure high-density culture: the bubbles do not directly contact with the cells, the cells can grow well in the fiber carrier, the inoculation density is low, the adherent cells are suitable for the serum-free culture, long time continuous perfusion, easy to realize continuous culture, long-term Harvest Cell Metabolic Product cell attached to the polyester tablets, and separated from the liquid, the harvest liquid can be from top to bottom, also can be from bottom to top, full use of carrier surface area, raise the culture density 9 scale-up using CFD calculation combined with physiological characteristic parameters to ensure scale-up reliability and feasibility of control system Biocore QC desktop Bioreactor developed on the PLC controller, the invention has the characteristics of stability, reliability and flexibility: 1. Heating, Ph, DO are related to stirring, and the transfer efficiency of eliminating local overheating, excessive acid and dissolved oxygen is low, to ensure the uniformity of all parameters in the tank 2, Ph control is controlled by feeding CO2 or adding Alkali, the addition of CO2 and Alkali is interlocking in a certain period of time to prevent excessive addition of CO2 and Alkali resulting in the change of the osmotic pressure of the culture solution, the DO control adopts step control mode and PID control mode, the control precision of dissolved oxygen is ± 1% 4, the control operation is simple, intuitive, easy to learn and use, high Flexibility 5, manual and automatic operation can be freely chosen, manual operation priority automatic operation, users can flexibly intervene in a link control process according to needs, user management adopts three-level authority management, different users have different Operation Authority, to prevent parameter tampering 7. The system can adopt electronic signature according to User Requirements, meet GMP requirements, technical parameter tank, total Volume 2L 5L 7.5 L 15L working volume 0.5-1.5 l 1.5-3.5 l 2-5 l 4-11L diameter to height ratio 1:1.8 material, borosilicate glass top cover PH/DO/inoculation/liquid transfer/temperature/shift/feed and other interface manufacturing standard ISO stirring, mode, top stirring, mechanical drive, Servo Motor, PID control display, digital display, ± 1rpm stirring speed 20 ~ 300rpm agitator, fixed bed agitator, pusher agitator, lifting agitator detector Mettler Hamilton, 0 ~ 12, Pid Control, accuracy: ± 0.01, with Alkali, CO2 CASCADE DO Mettler Hamilton, 0 ~ 200% , PID control, accuracy: ± 1% , with O2, N2, Air, flow, speed cascade liquid level, stainless steel probe, PID control temperature PT100, PID control, digital display, ± 0.1 °c gas control, flow measurement, hot Gas mass flow controller, ventilation 0.1 ~ 1l/min gas, four gas control system (N 2, O 2, Co 2, Air) , solenoid valve control intake filter 37mm 0.2 u, polytetrafluoroethylene exhaust filter 51mm 0.2 u, polytetrafluoroethylene gas condenser 316L stainless steel liquid flow, peristaltic pump, fixed function; Speed: 30rpm, 0 ~ 100% use duty ratio control temperature, control mode Pid, under the action of electric heating film temperature adjustment control precision, display precision: 0.1 °C, cONTROL ACCURACY: temperature control range ± 0.1 °C, tank size from 5 °C to 65 °C above ambient temperature, overall size 310 * 695 mm 310 * 695 mm 360 * 736 mm sterilization size 310 * 650 mm 310 * 650 mm 360 * 690 mm sterilization mode, off-line sterilization control system of autoclave, Siemens PLC system, cell Culture Program Data Record, man-machine interface, curve record, electronic signature matching remote monitoring (matching) , remote control (matching) water, electricity, gas requirements, cooling water pressure: Maximum 1kg, coarse filter 50um power supply: 220V, 50HZ, 10Amps input gas pressure: 2KG, external pressure gauge and regulator expansion unit, tail gas CO2/o2 Analyzer, Biomass on-line turbidity sensor, glucose concentration on-line detection, feed weighing, etc.

Click on the bioalpha Chinese website for more details on the application.