Product Intended use
This kit uses Real-time RT PCR technology (rRT-PCR) for qualitative detection of COVID-19 ( SARS-CoV-2 ) nucleic acids in human nasopharyngeal or oropharyngeal swab samples.
Principle of testing
This primer and probe sets are designed for the detection of the conserved sequences of COVID-19 (ORF1ab gene and N gene). The kit contains an Internal Control that is used to assess specimen quality.
Real-Time PCR technology utilizes polymerase chain reaction (PCR) for the amplification of specific target sequences and target specific probes for the detection of the amplified RNA. The probes are labelled with fluorescent reporter and quencher dyes.
Product characteristics
1.Quick and easy
Operator-friendly, without tedious nucleic acid purification. 96 samples can be
completed in 1.5 hour, suitable for large-scale screening.
2.Aviod nucleic acid loss
Samples can be directly lysed and released without purification steps, effectively
avoiding nucleic acid loss.
3.High virus loading
The loading of virus accounts for 1/20 of the virus preservation solution, higher
than other commercial kits.
4.Low equipment requirement and highly flexible use
No need for other equipment, it only requires Real-time PCR instrument and
centrifuge.
5.Low detection limit and high sensitivity
1) Detection sensitivity can reach the lowest level as 50 copies / reaction
2) The multiplex PCR detection of 50 copies of positive template, two pairs of test genes are both reported to be positive
Storage
- The kit should be stored at -20 ± 5℃ and protected from light. The validity period is 6 months.
- Avoid repeated freeze-thaw cycles (<5 cycles).
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