Human Dedicator of cytokinesis protein 3 (DOCK3) ELISA Kit,Abbkine_specification/price/image

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Dock3 was originally discovered in a screen for proteins that bind presenilin (a transmembrane protein which is mutated in early onset Alzheimer's disease).Dock3 is specifically expressed in neurones (primarily in the cerebral cortex and hippocampus).Dock3 is part of a large class of proteins (GEFs) which contibrute to cellular signalling events by activating small G proteins. In their resting state G proteins are bound to Guanosine diphosphate (GDP) and their activation requires the dissociation of GDP and binding of guanosine triphosphate (GTP). GEFs activate G proteins by promoting this nucleotide exchange.Dock3 exhibits the same domain arrangement as Dock180 and these proteins share a considerable (40%) degree of sequence similarity.

Human Dedicator of cytokinesis protein 3 (DOCK3) ELISA Kit employs a two-site sandwich ELISA to quantitate DOCK3 in samples. An antibody specific for DOCK3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDOCK3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DOCK3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DOCK3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Dedicator of cytokinesis protein 3 (DOCK3) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Find more details at http://www.abbkine.com/product/human-dedicator-of-cytokinesis-protein-3-dock3-elisa-kit-kte61991/

Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.