Human Ghrelin O-acyltransferase (MBOAT4) ELISA Kit,Abbkine_specification/price/image

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GOAT could modify ghrelin ser3 with fatty acids up to tetradecanoic acid. Replacement of his338 of GOAT with ala completely abolished the ability of GOAT to octanoylate ghrelin.mouse Goat octanoylated ghrelin (GHRL), a 28-amino acid appetite-stimulating peptide hormone, following cotransfection of Goat and preproghrelin in cultured endocrine cell lines. Mutation analysis showed that Goat octanoylated ghrelin on ser3, a modification required for its endocrine effects. Asp307 and his338 of Goat were required for octanoylation.The mouse and human proteins both contain 435 amino acids and have 8 putative transmembrane segments and conserved catalytic asparagine and histidine residues. Semiquantitative PCR of mouse tissues detected highest Goat expression in stomach, with lower expression in small intestine, colon, and testis.

Human Ghrelin O-acyltransferase (MBOAT4) ELISA Kit employs a two-site sandwich ELISA to quantitate MBOAT4 in samples. An antibody specific for MBOAT4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMBOAT4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MBOAT4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MBOAT4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Ghrelin O-acyltransferase (MBOAT4) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.