DNA replication licensing factor MCM2 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins.
Human DNA replication licensing factor MCM2 (MCM2) ELISA Kit employs a two-site sandwich ELISA to quantitate MCM2 in samples. An antibody specific for MCM2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMCM2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MCM2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MCM2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human DNA replication licensing factor MCM2 (MCM2) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Find more details at http://www.abbkine.com/product/human-dna-replication-licensing-factor-mcm2-mcm2-elisa-kit-kte61692/
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