ChIP Validated Antibody & Positive Primer Sets

Chromatrap^® ChIP-validated Antibodies can now be supplied with the correct matching primer sets to cover all aspects of ChIP validation.

•Reduce time spent searching databases and designing forward and reverse primers
•Purchase superior quality ChIP-validated antibodies with their matching high-quality primers
•Have confidence that the primers are tested with the exact antibody and gene target

The Chromatrap^® ChIP Validated Antibody Primer sets include a positive control antibody, a negative control antibody (non immune IgG) to evaluate non-specific binding and species-specific positive control qPCR primers.

ChIP Validated H3K27ac Antibody with Positive Primer Set
Product Code : 900021

Chromatrap^®’s ChIP Validated H3K27ac Antibody and Positive Primer Set provides a complete set of tools to assist with a successful ChIP assay. Including: H3K27ac antibody, control rabbit IgG, and a positive primer set. The ChIP Validated H3K27ac Antibody and Positive Primer Set is not suitable for use with non-human species.

Background:

Histone 3 (H3) is one of the core histone proteins, comprising the protein component of chromatin. H3 is ubiquitous within chromosomes and can be found bound to most gene sequences throughout the genome. Acetylation of histone H3 occurs at several different lysine positions on the histone tail and is catalysed by a group of enzymes called histone acetyltransferases (HATs). The acetylation of lysine 27 on histone 3 can be used to identify active enhancer sites, separating them from poised enhancers. Histone acetylation is associated with active transcription through its roles in chromatin remodelling and gene activation.

A rabbit IgG is included in this Antibody Primer Set as a negative control for the ChIP experiment.

The H3K27ac positive primer set recognises the promoter of a gene associated with active transcription and is a suitable target for this antibody.

•Applications: ChIP
•Source: Rabbit
•Concentration: 1mg/ml
•Type: Monoclonal
•Size: 50μl
•Purification: Protein A (affinity purified)
•Specificity: Human
•Storage Conditions: The H3K27ac antibody should be stored at +4°C. Rabbit IgG and the positive primer set should be stored at -20°C (Avoid multiple freeze/thaw cycles as this may denature the antibody and degrade the primer sets)