1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Ceftiofur in the tissue, liver, honey, intestine, shrimp, fish, milk, etc. The coupling antigens are pre-coated on the micro-well stripes. The Ceftiofur in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Ceftiofur antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with Ceftiofur concentration in the sample. This value is compared to the standard curve and the content of the corresponding Ceftiofur is subsequently obtained.
2. Technical specifications
Sensitivity : 1 ppb Detect time: Approx. 75 min
Detection limit:
Tissue..............................4 ppb
Milk ..............................40 ppb
Recovery rate:
Tissue, milk.................85 ±10%
Cross-reaction rate:
Ceftiofur ....................100%
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