1. General
Aflatoxin is a kind of fungus toxic metabolites. They mainly exist in the peanut, grains, nuts, seeds and some food, animal feed and other related products. Natural pollution of aflatoxin is given priority to aflatoxin B1,which is classified as first-degree carcinogen by WHO is the most toxic . It proved has great harm to human and animal liver, kidneys and other tissues and organs . Aflatoxin contamination can occur in plant growth, harvest and processing, storage, transportation in the process. The timely detection of pollution sources is the best way to prevent aflatoxin contamination.
2. Intended Use
A simple and efficient extraction and purification procedure for Aflatoxins was developed by means of the immunoaffinity column (IAC-SEP® AFLA) as a cleanup tool．Aflatoxins content in Grain, Food, Feeds, Nuts, Peanuts, Soy sauce, Vinegar, Chili, Pepper, Medicinal herbs and Wine samples are cleaned up by IAC and determined by HPLC or LC-MS/MS. It is a fast, simple, safe and highly accurate method for quantitatively measuring aflatoxins(B1、B2、G1、G2).
3. Principle
Samples are prepared by mixing with an extraction solution, blending and filtering. The extract is then applied to the Aflatoxins immunoaffinity column bound with specific antibodies to Aflatoxins．At this stage, the Aflatoxins bind to the antibody on the column. The column is then washed with water to remove the impurities. By passing methanol through the column, the Aflatoxins are removed from the antibody. This methanol solution can then be injected into HPLC or LC-MS/MS system.
ECALBO CO., LTD
Web: www.ecalbio.com
Address: HZAU, Nanhu Road, Wuhan city 430075, China.
Tel: +86 27 87745856
Fax: +86 27 87745856
E-mail: info@ecalbio.com
bio-equip.cn
中文
