1. General
T-2 producing fungi commonly attack grains and can grow at temperatures from slightly above freezing to about 30°C. All domestic animals are susceptible to injury by dietary intake of T-2 in the range of a few ppm. In poultry, feed contaminated with 1.0 to 3.5 ppm of T-2 has produced lesions at the edges of the beaks, abnormal feathering in chicks, a drastic and sudden drop in egg production, eggs with thin shells, reduced weight gains, and mortality.
2. Intended Use
A simple and efficient extraction and purification procedure for T-2 toxin was developed by means of the immunoaffinity column (IAC-SEP® T-2) as a cleanup tool．T-2 toxin content in grain, food, feed, nuts, peanuts, soy sauce, vinegar, chili, pepper, medicinal herbs and wine samples are cleaned up by IAC and determined by HPLC or LC-MS/MS. It is a fast,simple, safe and highly accurate method for quantitatively measuring T-2 toxin.
3. Principle
Samples are prepared by mixing with an extraction solution, blending and filtering.The extract is then applied to the T-2 toxin immunoaffinity column bound with specific antibodies to T-2 toxin．At this stage, the T-2 toxin bind to the antibody on the column.The column is then washed with water to remove the impurities.By passing methanol through the column, the T-2 toxin are removed from the antibody. This methanol solution can then be injected into HPLC or LC-MS/MS system.
Wuhan Ecalbio Co., Ltd
Web: www.ecalbio.com
Address: HZAU, Nanhu Road, Wuhan city 430075, China.
Tel: +86 27 87745856
Fax: +86 27 87745856
E-mail: info@ecalbio.com
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