This test kit is based on the competitive enzyme immunoassay for the detection of Rhodamine B in the Spice Oil,Oleoresins etc.
The coupling antigens are pre-coated on the microwell stripes.
The Rhodamine in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Rhodamine antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration.
The optical density (OD) value of the sample has a negative correlation with Rhodamine concentration in the sample.
This value is compared to the standard curve and the content of the corresponding Rhodamine is subsequently obtained.
Sensitivity : 0.2 ppb
Detect time: Approx.75 min
Detection limit:
Spice Oil,Oleoresins................. 200 ppb
Recovery rate:
Spice Oil,Oleoresins ................. 85±15%
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