Nuclease Detection Products,idt_specification/price/image

Nucleases are widely present in the laboratory environment and can interfere with many experiments.

In particular, single stranded RNases are ubiquitous, hard to eliminate, and can rapidly degrade

important samples used in microarray studies, real-time PCR, northern blots, or cDNA cloning.

IDT has developed reagents that allow for rapid, sensitive detection of RNases and DNases. These

reagents are fluorescence-quenched oligonucleotide probes that emit a fluorescence signal only

after nuclease degradation. The assay can be read visually or measured and quantified using

fluorometry. Assays can be used qualitatively to test lab reagents, equipment, and supplies for

nuclease contamination. Assays can be used quantitatively to study enzyme kinetics. Two

oligonucleotide substrates are available: one designed to detect RNases and the second designed

to detect DNases. The RNaseAlert® substrate employs a FAM™ reporter (Em 520 nm). The

DNaseAlert™ substrate employs a HEX™ reporter (Em 555 nm).

RNaseAlert and DNaseAlert are available as:

·Single-use tubes (25 tubes with 0.05 nmole each)—ideal for rapid hand held visual assays

at the research bench

·Bulk substrate (2 tubes with 2 nmole each)—for use in either visual assays or read in a fluorometer

·Kit—includes single use tubes, buffer, water, enzyme, and nuclease decontamination solution

RNaseAlert®

Kit Components:

·25 50 pmole Fluorescent Substrate tubes

·250 µL RNaseAlert® Buffer

·2 mL Nuclease-Free water

·50 µL RNase A

·50 mL Nuclease Decontamination Solution

·Kit quality guaranteed for six months from date received when stored at –20°C.

DNaseAlert™

Kit Components:

·25 50 pmole Fluorescent Substrate tubes

·250 µL DNaseAlert™ Buffer

·2 mL Nuclease-Free water

·25 µL 2U/mL DNase 1 Enzyme

·50 mL Nuclease Decontamination Solution

·Kit quality guaranteed for six months from date received when stored at –20°C.