Test Principle
Bovine Echinococcus Granulosus Antibody ELISA Test Kit is sandwich-antigen enzyme linked immunosorbent assay designed to detect the presence of antibody to Bovine Echinococcus Granulosus in the Bovine serum. A microtitration format has been configured by coating chlamydia pneumonia antigen in alternating columns on the plate. Upon incubation of the test sample in the coated well, antibody specific to Bovine Echinococcus Granulosus forms a complex with the coated viral antigens. After washing away unbound material from the wells, an anti-porcine horseradish peroxidase conjugate is added that binds to any Bovine antibody attached in the wells. In the final step of the assay, unbound conjugate is washed away, and enzyme substrate and a chromogen, TMB,are added to the wells. Stop the reaction by adding stop solution. Within 15 minutes READ the absorbance at 450 nm using 630 nm as the reference wavelength if available.
Enzyme immunoassays for the qualitative of antibodies against Bovine Echinococcus Granulosus in the Bovine serum or plasma.
Materials Supplied
1 Microtiter Plate 1 pieces (96 wells)
2 Negative control 1 tube
3 Positive control 1 tube
4 Enzyme Conjugate 1 bottle
5 Washing buffer 2 bottle
6 Substrate A 1 bottle
7 Substrate B 1 bottle
8 Stop solution 1 bottle
9 Sample diluent 2 bottle
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