Principle
Testosterone ELISA kit is a competitive enzyme immunoassay for the quantitative analysis of Testosterone in tissue, Cell media, Urine and Serum.
The coupling antigens are coated on the micro-well stripes. The Testosterone in the sample and the coupling antigens coated on the micro-well stripes compete for the anti-Testosterone antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration.
The optical density (OD) value of the sample has a negative correlation with the Testosterone in it. The measurement is made photometrically at 450 nm. The absorption is inversely proportional to the Testosterone concentration in the sample.
Technical specification
Sensitivity: 0.1 ppb Detect time: Approx.45 min
bio-equip.cn
中文
