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C-terminal Sequencing
C-terminal Sequencing
Place of Origin:
China
Brand:
Shanghai Applied Protein Technology Co. Ltd
Model:
Proteomics Services
Price:
Hits:
1235 
Updated:
5/9/2013
  • Product Detail
  • Company Profile
    Method:LC-MS

    Principle:
    Mass spectrometry (MS) is the technology of choice for rapidly identifying proteins present in a biological mixture. The primary advantages of MS are high sensitivity, accuracy, and capacity. Tandem mass spectrometry (MS/MS) provides a means for fragmenting mass-selected precursor peptide ions and measuring the mass-to-charge ratio (m/z) of any product daughter ions. The process usually produces two principle classes of fragment ions, the so-called N- and C-terminal b- and y-type ions respectively. High-quality MS/MS spectra of tryptic peptides typically show prominent b- and y-ion series.

    Equipment:
    Finnigan LCQ Deca Xp / Finnigan LTQ

    Samples Requirement:
    Salt concentration: Volatile salt<20mM, nonvolatile salt<5mM,Tris buffer is refused
    Protein amount: >50pmol; purity>95%

    Example:


    Result:
    C-terminal Sequencing: GSCNXXCVSGCAGRC (X represents some kind of amino acid, in secret)
    bio-equip.cn
    Shanghai Applied Protein Technology Co. Ltd is a biotech company to provide customer service and product development in the proteomics filed.

    The company is founded and owned by Research Center of Shanghai Institutes of Biological Sciences , Chinese Academy of Sciences.Our mission is to provide a total solution to our clients for their proteomics applications.

    Through the cooperation with the proteomics center of SIBS we have developed innovate technologies and proteomics research platforms to provide the best service for our clients in the basic research, drug discovery & development, molecular diagnostics and other life sciences areas. We are the contributor to the life science.
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