Principle: Confirm the structure of protein by matching theoratical sequence and peptides coverage
Equipment: Finnigan LCQ Deca Xp / Finnigan LTQ
Samples Requirement: 1. Salt concentration: Volatile salt<20mM, nonvolatile salt<5mM 2. Protein amount: >50pmol; purity>90%
Example:
Fig1: total ion chromatogram Fig2: peptides coverage
Result: Sequence of the sample is Ac-SDXXVDTXXEIXXKDLKEXXVVXXAEN (X represents some kind of amino acid, in secret)
bio-equip.cn
Shanghai Applied Protein Technology Co. Ltd is a biotech company to provide customer service and product development in the proteomics filed.
The company is founded and owned by Research Center of Shanghai Institutes of Biological Sciences , Chinese Academy of Sciences.Our mission is to provide a total solution to our clients for their proteomics applications.
Through the cooperation with the proteomics center of SIBS we have developed innovate technologies and proteomics research platforms to provide the best service for our clients in the basic research, drug discovery & development, molecular diagnostics and other life sciences areas. We are the contributor to the life science.