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QuickAntibody™-Mouse 5W Rappid Immune Adjuvant
Place of Origin:
China
Brand:
KBQ
Model:
Price:
$280
Hits:
1836 
Updated:
10/10/2013
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    QuickAntibody Adjuvant

    (English Version 2, 20120402)

    General Description

    QuickAntibody is a proprietary novel adjuvant designed specifically for the preparation of monoclonal as well as polyclonal antibodies in mice. Compared with the conventional Freund’s adjuvant, it has the unique features of fewer immunizations, less antigen dosage, quick antibody induction, higher antibody titers, higher antibody affinity, no destroy of natural antigen conformation, no epitope competition from other proteins, and easy to use.

    User Guidelines 

    1. QuickAntibody can achieve antigen-sparing effect through fewer immunizations (only two injections) and less antigen dosage per injection. Recommended antigen dosage is: (1) 5~20μg per injection for low immunogenic antigens such as recombinant subunit proteins or peptide-carrier conjugates; (2) 1~5μg per injection for high immunogenic antigens such as inactivated viruses or recombinant virus-like particles.

    2. QuickAntibody can induce a quick immune response, generating high titers as well as high affinity of antibodies. Whatever used for monoclonal as well as polyclonal antibody preparation, it requires only two immunizations within 3 weeks, and high titers of antibodies with ELISA titers up to 1:10000~1:10000000 could be elicited in the fifth week. 

    3. In contrast to Freund’s adjuvant, QuickAntibody doesn’t destroy natural antigen conformation, which facilitates the generation of monoclonal antibodies against conformational epitopes.

    4. In contrast to complete Freund’s adjuvant, QuickAntibody doesn’t have protein antigens, which eliminates the epitope competition from adjuvant-derived proteins.

    5. In contrast to the tedious emulsification required by Freund’s adjuvant, QuickAntibody is provided as a ready-to-use solution that can be easily mixed with any antigen before animal immunization.

    6. In contrast to the tedious foot-pad or intra-spleen immunization usually used in monoclonal antibody protocols, QuickAntibody can be conveniently used through intra-muscular injection.

    7. QuickAntibody is most useful in preparing murine polyclonal antibodies. In contrast to conventional rabbit polyclonal antibody preparation, which is usually provided by companies as customer-service, with QuickAntibody any laboratory can easily generate 1ml of high quality murine polyclonal antibodies within 5 weeks by simply immunizing 5 mice. (Note: 1ml of high quality murine polyclonal antibodies should fulfill most experimental requirements.)

    8. QuickAntibody is shipped at ambient temperature and stored at 2-8ºC (Do not freeze!). The adjuvant is stable for up to 6 months at 2-8ºC.

     

     

    Product Name: QuickAntibody-Mouse 5W

    Cat No:KX0210041

    Size1 ml

    Intended use: standard generation of murine monoclonal and polyclonal antibodies (ELISA titers up to 1:10000~1:1000000) by two immunizations within 5 weeks.

    Immunization Protocol

    1. Dilute the antigen with saline to 2-fold final concentration (50ul per injection).

    Note: Recommended antigen dosage is: (1) 5~20μg per injection for low immunogenic antigens such as recombinant subunit proteins or peptide-carrier conjugates; (2) 1~5μg per injection for high immunogenic antigens such as inactivated viruses or recombinant virus-like particles.

    2. Mix the adjuvant by vortexingsterilely remove the required volume (50ul per injection), and mix with the antigen at 1:1 ratio (V/V) as quick as possible.

    Note: It is normal to see precipitation in the adjuvant.

    3. Inject 100μl of the antigen/adjuvant mixture into a quadriceps muscle of each mouse. Note: It is normal to see precipitation in the antigen/adjuvant mixture. Please mix well before drawing into syringe and inject as quick as possible after drawing into syringe.

    4. Boost animals on day 21 post-priming.

    Note: For boosting, please follow steps 1-3 of the priming protocol, using freshly prepared antigen/adjuvant mixture.

    5. Bleed from tail tips on day 35 post-priming and measure the antibody titers by ELISA. ELISA titers should be in the range of 1:10000~1:1000000. After that the whole serum can be collected or proceed to antigen boost and cell fusion according to conventional hybridoma protocols.

    bio-equip.cn
    Beijing KANG BI QUAN Biotech Co., Ltd is a company specialized in the R&D of novel bio-chemical reagents. The company’s philosophy is: to create innovative brand products, to change conventional experimental methods, to make research more efficient and easier. The company currently has two product series:
    QuickAntibody™ is a proprietary novel adjuvant designed specifically for the preparation of monoclonal/polyclonal antibodies in mice and polyclonal antibodies in rabbits. Compared with the conventional Freund’s adjuvant, it has the unique features of fewer immunizations, less antigen dosage, quick antibody induction, comparable antibody titers, comparable antibody affinity, no destroy of natural antigen conformation, no epitope competition from other proteins, and ease of use.
    QuickShuttle™ is a proprietary cationic polymer-based transfection reagent, which is optimized for the purpose of maximal transfection efficiency, ease of use, and minimal cytotoxicity. It is recommended for plasmid DNA transfection into mammalian cells by means of transient transfection as well as stable cell line generation. QuickShuttle™ has two unique features that other conventional transfection reagents don’t have: (1) transfection could be done immediately after cell subculture; (2) transfection could be completed in just one minute.
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