Adhere Mix Mode Anion Exchange Resin

Adhere Mix Mode Anion Exchange Resin use polymethacrylate as the matrix. It is a multimodal chromatography resin with anion exchange, hydrophobic and hydrogen bonding interactions, specifically designed for monoclonal antibody purification and polishing. The product features high flow rate, high binding capacity, and excellent chemical stability and physical properties. It can remove protein A, aggregates, host proteins, nucleic acids and viruses in a single step which improves efficiency and saves costs.

2.Features

Chromatographic operation Procedure

Chromatographic operation typically includes steps such as equilibration, sample loading, washing, elution, and regeneration. The specific operating methods are as follows:

Equilibration: Equilibrate the column with 5-10 column volumes (CV) of equilibration buffer (e.g., 20 mM PB + 0.1 M NaCl, pH7.0; the specific buffer system should be selected and optimized based on the stability and hydrophobicity of the target protein) at a flow rate not exceeding the maximum pressure limit, until the conductivity and pH of the effluent remain constant (consistent with the equilibration buffer).

Sample Loading: The sample loading buffer should be as consistent as possible with the equilibration buffer; solid samples can be dissolved and prepared in equilibration buffer; dilute samples can be dialyzed with equilibration buffer or supplemented with the corresponding amount of Solution composition; concentrated sample solutions can be diluted with equilibration buffer. To avoid clogging the chromatography column, sample solutions should be centrifuged or microfiltered (0.45 μm). The loading volume should be calculated based on the binding capacity and the target protein content in the sample solution.

Washing: After loading, continue washing with equilibration buffer until the UV absorbance drops to baseline.

Elution: Elute with elution buffer (e.g., 20 mM PB+1M NaCl, pH7.0). Elution methods include pH gradient elution, linear gradient elution, or step gradient elution.

Regeneration: After each cycle, clean the column with 1-2M NaCl to Remove strong binding proteins.

Cleaning In Place (CIP): After 5-10 uses (the specific number depends on the type and source of raw materials and experimental requirements), the column requires Cleaning In Place, which can refer to the table below.

Rinse immediately with 5CV of equilibration buffer after CIP completion.

Storage: Store in 20% ethanol at 2-30°C; The unpacked resins can be flushed with 20% ethanol and then stored at 2-30°C.

Other Considerations: When using and storing the column, avoid letting it run dry or become poorly sealed to prevent air bubbles from entering.
3.Specification