High-pressure homogenizer is a critical piece of equipment that utilizes ultra-high pressure to force materials through narrow gaps at high velocity, creating physical effects such as shear, impact, and cavitation. This enables cell disruption, particle size reduction, and fluid homogenization. Key operational parameters include homogenization pressure, number of passes, and processing temperature, all of which can be flexibly adjusted according to process requirements.

In the biopharmaceutical industry, high-pressure homogenizers are widely used for cell disruption and extraction in E. coli, yeast, and mammalian cells; the preparation of recombinant proteins and vaccine antigens; and the homogenization of nanoparticles. By optimizing process conditions, high-pressure homogenization can achieve efficient and highly reproducible processing while maintaining the integrity of active products.

Duoning Biotech’s high-pressure homogenizers are specially engineered for bioprocess optimization, offering outstanding cell disruption efficiency and precise particle size control. The equipment covers the full spectrum from lab-scale development to industrial-scale production. Large-scale systems can be equipped with intelligent control software for parameter recording and traceability, ensuring batch-to-batch consistency and process stability. The structural design of Duoning’s homogenizer series emphasizes durability and ease of maintenance, providing robust support to enhance process efficiency and accelerate R&D and time-to-market for customers.

Laboratory and Pilot-Scale High-Pressure Homogenizers

High-pressure homogenizers from lab to pilot scale are designed to support a wide range of applications, from early-stage research to process scale-up. The DH-Basic and DH-Nano series are specifically developed for laboratory use, offering a minimum processing volume as low as 25 mL and flexible operation. The DH-Pilot series is ideal for pilot-scale applications, with a flow rate of up to 40 L/h, making it suitable for continuous production needs. All models are equipped to handle working pressures up to 1500 bar, featuring compact design and stable performance—empowering efficient cell disruption and nano-scale homogenization processes.

DH-Basic	DH-Nano	DH-Pilot

• Pressure up to 1500 bar, suitable for a variety of biological sample disruption needs

• Flexible flow rate ranging from 5 – 40 L/H, balancing both R&D and scale-up requirements

• Pneumatic automatic control version equipped with a 10-inch touchscreen and high-precision pressure sensors, ensuring ease of operation and data traceability

• Built-in heat exchanger to maintain stable temperature during processing

• Standard zirconia plunger, wear-resistant and corrosion-resistant, ensuring long-term operational stability

• Optional yeast-specific homogenizing valve to optimize specific process requirements

• Optional chiller available to support high-frequency operation needs

• Includes maintenance tools and commonly used seals, ensuring ease of use and worry-free after-sales service