1. Principle
This test kit is based on the indirect competitive enzyme immunoassay for the detection of total Aflatoxin. The coupling antigen is pre-coated on the micro-well stripes. The Aflatoxinin the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti- Aflatoxinantibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Aflatoxinin the sample. This value is compared to the standard curve and the Aflatoxin residues is subsequently obtained.
2. Technical specifications
Sensitivity: 0.02 ppb
Incubator temperature: 25℃
Incubator time: 30min~15min
Detection limit:
Feed, rice, corn, peanut, tissue, edible oil……………………………….2ppb
Cross-reaction rate:
Aflatoxin B1…..............................................................................….100%
Aflatoxin M1…............................................................................. …91.2%
Aflatoxin B2…............................................................................. ….68.4%
Aflatoxin G1….............................................................................. ….4.7%
Aflatoxin G2….............................................................................. ….2.7%
Recovery rate:
Feed, rice, corn, peanut, tissue, edible oil………………………………95±35%

We are Shenzhen Lvshiyuan Biotechnology Co.,Ltd (www.lsybt.com), professional manufacturer and exporterof the most advanced Food Safety ELISA test kit(e.g. Nitrofuran (AMOZ, AOZ, AHD, SEM) ELISA kit, Clenbuterol ,Ractopamine ELISA kit), Animal Disease Diagnostic kits(e.g. Swine FMD lgG Distinguish kit,PRRS,PRV ELISA kit, NDV,IB,IBD ELISA kit),small molecule antigen and antibodies, gene recombination antigen and antibodies, etc.
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